| HPLC法测定林下山参制浆前后人参皂苷Re、Rg_1、Rb_1、Rg_3、Rh_1、Rh_2含量的变化 |
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| 引用本文： | 闫彤,石国慧,吴韶,肖婷,沈光海,赵余庆.HPLC法测定林下山参制浆前后人参皂苷Re、Rg_1、Rb_1、Rg_3、Rh_1、Rh_2含量的变化[J].中草药,2018,49(17):4148-4151. |
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| 作者姓名： | 闫彤 石国慧 吴韶 肖婷 沈光海 赵余庆 |
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| 作者单位： | 延边大学药学院;沈阳药科大学功能食品与葡萄酒学院;沈阳药科大学基于靶点的药物设计与研究教育部重点实验室 |
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| 基金项目： | 天然创新药物原料及提取物工业化色谱制备工程研究中心资助项目 |
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| 摘 要： | 目的分析林下山参制浆后人参皂苷Re、Rg_1、Rb_1、Rg_3、Rh_1、Rh_2含量的变化情况。方法采用HPLC-UV法,Innoval ODS-2色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-水溶液进行梯度洗脱;柱温30℃;体积流量1.0 mL/min;进样体积20μL;检测波长203 nm。结果林下山参中6种人参皂苷Re、Rg_1、Rb_1、Rg_3、Rh_1、Rh_2质量分数分别由制浆前的0.651、0.506、0.363、0.014、0.023、0.031 mg/g变化为制浆后的0.517、0.413、0.105、0.122、0.214、0.098 mg/g。人参皂苷Re、Rg_1、Rb_1、Rg_3、Rh_1、Rh_2分别在2.5~100 mg/L具有良好的线性关系,r2均大于0.999 5;精密度、稳定性及重复性良好;平均加样回收率为95%~105%,RSD为1.25%~3.05%。结论林下山参中6种人参皂苷Re、Rg_1、Rb_1、Rg_3、Rh_1、Rh_2的含量在制浆前、后发生变化。林下山参制浆后人参皂苷Re、Rg_1、Rb_1的含量降低,稀有人参皂苷Rg_3、Rh_1、Rh_2的含量分别增高8.7、9.3、3.2倍。基于HPLC最佳分离参数建立的6种人参皂苷成分同时分析的方法具有较好的准确性和可靠性,可为林下山参浆的质量评价提供科学依据。
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| 关 键 词： | 林下山参浆 人参皂苷Re 人参皂苷Rg1 人参皂苷Rb1 人参皂苷Rg3 人参皂苷Rh1 人参皂苷Rh2 HPLC |
| 收稿时间： | 2018/3/6 0:00:00 |
Determination of content changes of ginsenoside Re, Rg1, Rb1, Rg3, Rh1, and Rh2 before and after pulping of mountain cultivated ginseng by HPLC |
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| YAN Tong,SHI Guo-hui,WU Shao,XIAO Ting,SHEN Guang-hai and ZHAO Yu-qing.Determination of content changes of ginsenoside Re, Rg1, Rb1, Rg3, Rh1, and Rh2 before and after pulping of mountain cultivated ginseng by HPLC[J].Chinese Traditional and Herbal Drugs,2018,49(17):4148-4151. |
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| Authors: | YAN Tong SHI Guo-hui WU Shao XIAO Ting SHEN Guang-hai and ZHAO Yu-qing |
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| Affiliation: | School of Pharmacy, Yanbian University, Yanji 133002, China,School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China,School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China,School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China,School of Pharmacy, Yanbian University, Yanji 133002, China and School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang 110016, China;Key Laboratory of Structure-based Drug Design and Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | |
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| Keywords: | mountain cultivated ginseng pulp ginsenoside Re ginsenoside Rg1 ginsenoside Rb1 ginsenoside Rg3 ginsenoside Rh1 ginsenoside Rh2 HPLC |
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