鸦胆子油抗肿瘤作用时间药理学的研究

目的:探索鸦胆子油抗肿瘤作用的时辰用药规律,为临床肿瘤治疗学提供一定的实验依据。方法:昆明种小鼠72只,雌雄各半,分成6组,分别为正常组,模型组,环磷酰胺组(20 mg·kg-1),鸦胆子油上午6点给药组,下午2点给药组,下午10点给药组(6 am给药组,2 pm给药组,10 pm给药组),鸦胆子油ip鸦胆子油乳5 m L·kg-1,除正常组外,制备肝癌H 22荷瘤小鼠动物模型,连续给药10 d后,眼球取血,分离血清,测定丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),总蛋白(TP),尿素氮(BUN)和血肌酐(SCr)等肝肾评价指标,脱颈椎处死,刺离肿瘤、胸腺,脾脏,肾脏、肝脏,称质量,计算抑瘤率及其脏器指数,并采用光镜观察肉瘤组织病理变化。结果:与模型组比较,鸦胆子油6 am,2 pm,10 pm给药组能显著抑制瘤重(P0.01),抑瘤率分别为34.98%,39.92%,42.80%,能够显著提高肝、肾、脾、胸腺的指数(P0.01),以2 pm,10 pm给药组作用最好,能够降低ALT,AST,TP,UNB,SCr的含量,其中10 pm给药组ALT,TP,SCr的含量降低最为明显(P0.05,P0.01),2 pm给药组AST,BUN含量降低最为明显(P0.05,P0.01);组织病理结果显示,模型组肉瘤组织坏死区域较大,3个给药组均可减少肉瘤细胞坏死区,以10 pm给药组最为明显。结论:鸦胆子油具有一定的抗肿瘤和保护肝肾的作用,而其疗效和毒副作用受给药时间的影响,以10 pm给药效果最佳,副作用较少。

The Research of Time Pharmacology of Anti-tumor Effect for Bruceae Fructus Oil

Objective: To explore the medication rules of Bruceae Fructus oil for anti-tumor effect and provide some experimental basis for clinical treatment of cancer. Method: Seventy-two Kunming mice, half male and half female, were divided into 6 groups:normal group, model group, cyclophosphamide group (20 mg·kg^-1), Bruceae Fructus oil 6 am administration group, 2 pm administration group, and 10 pm ministration group. Bruceae Fructus oil groups received oil emulsion 5 mL·kg^-1. All other groups except normal group prepared hepatoma H22 tumor-bearing mice as animal models. After 10 d of continuous oral administration, blood samples were taken from eyeball and serum was separated to determine the content of alanine amino transferase(ALT), aspartate amino transferase(AST), total protein(TP), urea nitrogen(UNB), serum creatinine(SCr) and other liver and kidney evaluation indexes. Mice were sacrificed by cervical dislocation, and their tumors, thymus, spleen, kidney, and liver were isolated for weighing. The tumor inhibitory rate and organ index were calculated then, and light microscopy was used to observe histopathological changes of sarcoma tissues. Result: Compared with model group, Bruceae Fructus oil 6 am, 2 pm, and 10 pm groups could significantly inhibit tumor weight (P<0.01) and its inhibition rate was 34.98%, 39.92% and 42.80% respectively, and could significantly improve the liver, kidney, spleen, and thymus indexes (P<0.01). 2 pm, 10 pm administration groups achieved best results, and could reduce the content of ALT, AST, TP, BUN, SCr, wherein 10 pm administration group could most reduce ALT, TP, and SCr (P<0.05, P<0.01), and 2 pm administration group could most reduce AST, BUN (P<0.05, P<0.01). The histopathological results showed that the model group had larger necrosis area for sarcoma tissues, and three treatment groups can reduce necrosis area of sarcoma cells, with 10 pm administration group most obvious. Conclusion: Bruceae Fructus oil has some anti-tumor effect and protects the liver and kidney function, and its efficacy and side effects were affected by administration time. 10 pm administration group has the best results and fewer side effects.