双(α-呋喃甲酸)氧钒对胰岛素抵抗3T3-L1脂肪细胞糖摄取的影响

目的探讨新型的有机羧酸氧钒配合物双(α-呋喃甲酸)氧钒(BFOV)对正常及胰岛素抵抗的3T3-L1脂肪细胞糖摄取的影响。方法采用地塞米松诱导3T3-L1脂肪细胞建立胰岛素抵抗的细胞模型,研究双(α-呋喃甲酸)氧钒对正常及胰岛素抵抗3T3-L1脂肪细胞葡萄糖消耗的影响。结果双(α-呋喃甲酸)氧钒(2.5μmol·L-1~40μmol·L-1)对正常的3T3-L1脂肪细胞仅有增加葡萄糖消耗量的趋势,与空白对照组比较,差异无显著性;但能明显增加地塞米松诱导的胰岛素抵抗3T3-L1脂肪细胞的葡萄糖消耗量,改善模型细胞的胰岛素抵抗状态。结论双(α-呋喃甲酸)氧钒能促进胰岛素抵抗脂肪细胞的葡萄糖摄取,改善胰岛素抵抗状态。

Effects of bis(α-furancarboxylato)oxovanadium(IV) on the glucose uptake in normal and insulin-resistant 3T3-L1 adipocytes

Aim To investigate the effects of bis(α-furancarboxylato)oxovanadium(IV),BFOV] on the glucose uptake in normal and insulin-resistant 3T3-L1 adipocytes.Method Fully differentiated 3T3-L1 adipocytes were treated with dexamethasone to induce insulin resistance.Effects of bis(α-furancarboxylato)oxovanadium(IV) on the glucose uptake in normal and insulin-resistant adipocytes were estimated by the changes of glucose consumption in the medium measured by the glucose oxidase method.Results BFOV(2.5～40 μmol·L-1) produced the tendency of increasing the glucose consumption in normal cells,compared with untreated control cells(P>0.05).However,BFOV markedly enhanced the glucose consumption in insulin-resistant 3T3-L1 adipocytes,resulting in the improvement of insulin resistance induced by dexamethasone.Conclusion BFOV increased the glucose uptake in insulin-resistant adipocytes,suggesting the improvement of insulin resistance.