| HPLC法测定假奓包叶叶中没食子酸的含量 |
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| 引用本文： | 陈晟,张元媛,赵敏,张璐,王军宪.HPLC法测定假奓包叶叶中没食子酸的含量[J].西北药学杂志,2009,24(3). |
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| 作者姓名： | 陈晟 张元媛 赵敏 张璐 王军宪 |
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| 作者单位： | 西安交通大学医学院药学系,陕西,西安,710061 |
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| 摘 要： | 目的测定假奓包叶叶中没食子酸的含量。方法采用Dikma Diamonsil C18柱(250mm×4.6mm,5μm),以甲醇-0.1%磷酸水溶液(5∶95)为流动相,流速1.0mL·min-1,柱温25℃,检测波长为269nm,以外标法测定假奓包叶叶中没食子酸的含量。结果没食子酸在0.0044～0.2180g.L-1质量浓度范围内呈线性关系(r=0.9996),平均回收率为100.6%(n=5)。结论该方法简便、准确,重现性好,可用于假奓包叶中没食子酸的含量测定。
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| 关 键 词： | 假奓包叶 没食子酸 含量测定 高效液相色谱法 |
HPLC determination of gallic acid in the leaves of Discocleidion rufescens |
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| CHEN Sheng,ZHANG Yuanyuan,ZHAO Min,ZHANG Lu,WANG Junxian.HPLC determination of gallic acid in the leaves of Discocleidion rufescens[J].Northwest Pharmaceutical Journal,2009,24(3). |
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| Authors: | CHEN Sheng ZHANG Yuanyuan ZHAO Min ZHANG Lu WANG Junxian |
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| Abstract: | Objective To assay gallic acid in the leaves of Discocleidion rufescens by HPLC.Method A Dikma Diamonsil C18 column(250 mm×4.6 mm,5 μm)was used with a mobile phase of methanol-water(0.1% phosphoric acid)(5:95)at a flow rate of 1.0 mL·min-1.In this method,the column temperature was 25 ℃,and UV detector wavelength was 269 nm.Results The linear range of gallic acid was 0.004 4~0.218 0 g·L-1(r=0.999 6).The average recovery was 100.6%(n=5).Conclusion A simple and accurate quantitative method with good reproducibility to assay gallic acid in the leaves of Discocleidion rufescens has been established. |
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| Keywords: | Discocleidion rufescens gallic acid quantitative determination HPLC |
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