Livin siRNA重组腺病毒对胶质瘤U251细胞的增殖、凋亡的作用研究

目的通过小分子干扰RNA(siRNA)沉默livin的表达，探讨其对胶质瘤U251细胞增殖及凋亡的影响。方法制备livin siRNA重组腺病毒后感染U251细胞,采用RT-PCR、蛋白质印迹法检测livin基因的干扰效果,用MTT法和流式细胞术检测U251细胞的增殖和凋亡情况。同时观察重组腺病毒对荷瘤小鼠的治疗效应。结果重组腺病毒Ad-livin siRNA能有效抑制U251细胞的livin mRNA和蛋白水平的表达；抑制U251细胞增殖,促进凋亡；在小鼠体内有效抑制U251细胞的生长和提高荷瘤小鼠的平均生存期（P<0.05）。结论用livin siRNA重组腺病毒介导抑制livin基因的表达,可抑制U251细胞增殖,促进凋亡，为胶质瘤的治疗提供了新策略。

The effect study of recombinant adenovirus Livin siRNA on the proliferation and apoptosis of U251 cells

ObjectiveTo investigate the silencing effect of siRNA targeting livin gene on the proliferation and apoptosis of U251 cells. MethodsAdenoviral vector of siRNA targeting livin was constructed and transfected into U251 cells. The gene silencing efficiency was monitored by RT-PCR and Western blotting analysis; the proliferation and apoptosis of U251 cells were detected by MTT assays and flow cytometry, respectively. Meanwhile, the therapeutic effect of the recombinant adenovirus was also observed in tumor-bearing mice. ResultsThe constructed recombinant adenovirus effectively inhibited the expression of livin at both mRNA and protein levels; it also inhibited the proliferation and promoted the apoptosis of U251 cells. In addition, the recombinant adenovirus effectively inhibited the tumor growth in mice and improved the mean survival period of tumor-bearing mice. ConclusionSilencing livin gene expression with recombination adenovirus carrying siRNA targeting livin can inhibit the proliferation and promote the apoptosis of U251 cells, which casts new lights on the treatment of glioma.