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| p38MAPK基因诱导胶质瘤细胞凋亡 | |
| 引用本文： | 章必成,李青,叶菁,陈广生,王映梅,巩西启,林圣彩.p38MAPK基因诱导胶质瘤细胞凋亡[J].第四军医大学学报,2001,22(3):211-213. |
| 作者姓名： | 章必成 李青 叶菁 陈广生 王映梅 巩西启 林圣彩 |
| 作者单位： | 1. 第四军医大学西京医院病理科, 2. 北京卫戍区三师医院,北京 3. 新加坡国立大学分子细胞生物学研究所,新加坡 |
| 基金项目： | 高等学校骨干教师计划资助 |
| 摘 要： | 目的 研究p38MAPK基因转染大鼠胶质瘤细胞系C6后对其生物学特性的影响。方法 利用脂质体介导法将p38MAPK基因导入大鼠胶质瘤细胞系C6中，用免疫细胞化学染色检测其在细胞转染前后的表达情况，用HE染色、流式细胞仪等方法研究其对细胞形态、粘着状况和生长周期的影响。结果 转染pCMV5-P38MAPK质粒组p38MAPK蛋白表达阳性，细胞形态发生变化，贴壁性降低，出现大量凋亡细胞。结论 转染p38MAPK基因可诱导胶质瘤细胞凋亡。 |
| 关 键 词： | p38MAPK基因 基因转染 胶质瘤 细胞凋亡 |
| 文章编号： | 1000-2790(2001)03-0211-03 |
| 修稿时间： | 2000年6月14日 |
p38MAPK induces apoptosis of glioma cell |
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| ZHANG Bi Cheng ,LI Qing ,GONG Xi Qi ,YE Jing ,CHEN Guang Sheng ,WANG Ying Mei ,LIN Sheng Cai.p38MAPK induces apoptosis of glioma cell[J].Journal of the Fourth Military Medical University,2001,22(3):211-213. | |
| Authors: | ZHANG Bi Cheng LI Qing GONG Xi Qi YE Jing CHEN Guang Sheng WANG Ying Mei LIN Sheng Cai |
| Affiliation: | ZHANG Bi Cheng 1,LI Qing 1,GONG Xi Qi 2,YE Jing 1,CHEN Guang Sheng 1,WANG Ying Mei 1,LIN Sheng Cai 3 1Department of Pathology,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China,2Third Division Headqua |
| Abstract: | AIM To study the effect of p38MAPK transfection on the biological characteristics of glioma cell C6. METHODS p38MAPK was transfected into glioma cell C6 by lipofectin. Expression of p38MAPK was detected by immunocytochemistry. HE staining and flow cytometry were adopted to measure the cell morphology, adhesion and cell cycle. RESULTS p38MAPK was expressed in transfected glioma cells, with cell biological characteristics changing and apoptotic cells emerging. CONCLUSION Apoptosis of glioma cell could be induced by p38MAPK transfection. |
| Keywords: | p38MAPK gene transfection glioma apoptosis rats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
