| 不同地区不同生境按蚊种群的分子鉴定及rDNA-ITS2序列分析 |
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| 引用本文： | 谭伟龙,赵彤言,董言德,汪中明,李春晓.不同地区不同生境按蚊种群的分子鉴定及rDNA-ITS2序列分析[J].寄生虫与医学昆虫学报,2011,18(2):84-89. |
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| 作者姓名： | 谭伟龙 赵彤言 董言德 汪中明 李春晓 |
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| 作者单位： | 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071;南京军区疾病预防控制中心,南京,210002 |
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| 摘 要： | 对采自安徽、江苏、湖南、广西、云南及海南等地区的人房、牛棚、稻田、山区等不同生境的按蚊,按照文献合成引物,用PCR方法进行分子鉴定;并对各种群的ITS2序列测序后,与GenBank所得赫坎按蚊复合体近缘种群按蚊的ITS2序列进行比对分析.结果显示,采集的不同地区不同生境的按蚊均为中华按蚊,此次采集没有采到嗜人按蚊;现场...
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| 关 键 词： | 按蚊 分子鉴定 rDNA-ITS2 序列分析 同源性 |
Molecule identification and rDNA-ITS2 sequence analysis of Anopheles from different provinces and habitats |
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| TAN Wei-Long,ZHAO Tong-Yan,DONG Yan-De,WANG Zhong-Ming,LI Chun-Xiao.Molecule identification and rDNA-ITS2 sequence analysis of Anopheles from different provinces and habitats[J].Acta Parasitologica et Medica Entomologica Sinica,2011,18(2):84-89. |
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| Authors: | TAN Wei-Long ZHAO Tong-Yan DONG Yan-De WANG Zhong-Ming LI Chun-Xiao |
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| Affiliation: | TAN Wei-Long ZHAO Tong-Yan DONG Yan-De WANG Zhong-Ming LI Chun-Xiao (1. Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China; 2. Central of Disease Prevention and Control of Nanjing Military Area, Nanjing 210002, China) |
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| Abstract: | The partial rDNA-ITS2 segments of Anopheles from Jiangsu, Anhui, Hunan, Yunnan, Hainan provinces and Guangxi Zhuang autonomous region were amplified using PCR and then were sequenced. The sequence alignment results of rDNA-ITS2 indicated that all the samples obtained from different provinces and habitats belonged to Anopheles sinensis. There were high identities between the samples group which ranging from 98.2% to 100%. The identities between the samples and An. hycauns （An. liangshanensis, An. kunmingensis, An. lesteri and An. anrhropophagus, An. nigerrimus An. peditaeniatus） were 79. 1% -82% , 76. 1% -77. 1% , 60.9% -63.9% , respectively. Phylogenetic analysis results showed that all the segments obtained from the fields were clustered together and formed two separated branches with the segments of An. hycauns form GenBank. |
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| Keywords: | rDNA-ITS2 |
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