氧化石墨烯/丝素蛋白凝胶结构及其性能研究

目前医用凝胶材料普遍存在力学强度较差及生物降解速度过快等问题。以蚕丝丝素蛋白(SF)凝胶为基础材料,加入氧化石墨烯(GO),并将1-乙基-(3-二甲基氨基丙基)碳二亚胺盐酸盐(EDC)及N-羟基琥珀酰亚胺(NHS)作为交联剂制备SF/GO复合凝胶,旨在改善凝胶材料的力学性能,使其在保证复合凝胶材料应有的力学强度的同时,发挥丝素蛋白、氧化石墨烯的生物学效应及凝胶材料的多孔支架作用。实验结果显示,EDC的加入可以使得SF、GO共混形成稳定、均匀的无规卷曲结构,扫描电镜(SEM)显示SF/GO凝胶具有典型的多孔结构。GO的加入可以有效缩短复合材料的凝胶时间,同时复合凝胶材料的力学性能得到明显改善,其压缩强度提高40%以上。GO的加入还可明显延长材料的降解时间。基于SF/GO的复合凝胶在组织修复及再生领域具有较好的应用前景。

Fabrication and Characterization of Silk Fibroin/Graphene Oxide Composite Hydrogel

In the present study, the problems of poor mechanical strength and unsatisfactory biodegradation time is generally found in medical hydrogel materials.Thus,a new model of porous scaffolds for tissue engineering was described. Composite hydrogel based on silk fibroin(SF) and graphene oxide(GO) were fabricated by simply edding the chemical crosslinking agent.The composite hydrogel showed random coils according X-ray diffraction(XRD) and three-dimensional porous by scanning electron microscope(SEM).Compared with the SF hydrogel, the hydrogels prepared by incorporating GO showed a higher formation rate and a lower enzymatic degradation rate. Moreover, hydrogels exhibited favorable elasticity and compressive strength up to 140%.We suggest that the composite hydrogel will be one of the valuable material to future tissue engineering application.