Biohydrogenation of cholesterol as an index of bacterial 7α-dehydroxylase activity |
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Authors: | Charles T L Huang M M Levine W E Woodward G S Gopalakrishna D R Nalin R B Hornick B L Nichols |
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Affiliation: | (1) Section of Nutrition and Gastroenterolory, Department of Pediatrics, Baylor College of Medicine, 77030 Houston, TX;(2) Division of Infectious Diseases, University of Maryland, School of Medicine, 21201 Baltimore, MD;(3) Present address: Merichem Research Center, 77012 Houston, TX |
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Abstract: | Fecal steroid compositions of 82 human subjects of various ages and diets and gastrointestinal status were examined by gas
liquid chromatography. Progressive increases in bacterial activities on both bile acids and neutral sterols were observed
with the advance of age in infants and children. The patterns in the 4-year-olds approached those observed in adults. Bacterial
activites on fecal steroids were found to be decreased in adult subjects with acute shigellosis and in those challenged by
castor oil. In contrast, no significant changes in fecal steroid profiles were observed in the subjects with traveller's diarrhea
assoicated with toxigenicEscherichia coli. The effects of diarrhea on fecal steroids of infants under 11/2 years were less consistent than those of adults. However, a close relationship was observed between the degree of 7α-dehydroxylation
of cholic acid (expressed as the ratio of deoxycholic to the sum of deoxycholic and cholic acids) and the percentage of cholesterol
in the feces (r= 0.921, p<0.001). The correlation between the production of lithocholic acid and the percentage cholesterol
was also good (r=−0.739, p<0.001). Analysis of neutral steroids may be a good index of intraluminal bile acid metabolism. |
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