HPLC法测定人血浆中咪达唑仑的浓度

目的:建立以高效液相色谱法测定人血浆中咪达唑仑浓度的方法。方法:以安定为内标,色谱柱为Agilent Eclipse XDB C18,流动相为0.02mol·L-1NaH2PO4水溶液(pH7.6,内含0.02%三乙胺)-乙腈(52∶48),流速为1.0mL.min-1,检测波长为223nm,灵敏度为0.001AUFS,进样量为50μL,柱温为40℃。结果:咪达唑仑血浆浓度在10~1 600ng·mL-1范围内线性关系良好(r=0.999 1),最低检测限为1ng·mL-1;日内、日间RSD≤7.25%。结论:本方法简便、灵敏、准确、快速,检测范围广,可用于咪达唑仑的血药浓度监测及临床药动学研究。

Determination of Plasma Concentration of Midazolam by HPLC

OBJECTIVE： To establish an HPLC method for the determination of midazoalm in human plasma. METHODS： Human plasma with the internal standards （diazopam） was separated on Agilent XDB-C18 with a mobile phase of acetonitrile- 0.02 mol · L^-1NaH2PO4 （pH= 7.6, containing 0.02 mol · L^-1 TEA）（48 ： 52） at a flow rate of 1.0 mL · min^ -1 and detected at 223 nm. The sensitivity was 0.001 AUFS; the sample size was 50 μL and the column temperature was 40℃. RESULTS： The minimum detectable concentration of midazolam in plasma was 1 ng · mL^-1, and the calibration curve showed good linearity in a concentration range of 10-1 600 ng · mL^-1（r = 0.999 1）. The intra- day RSD and inter- day RSD were both less than 7.25% . CONCLUSION： The method is simple, sensitive, accurate and specific, and may be applied to the clinical pharmacokinetic study of midazolam.