芒果苷减轻IL-1β诱导的软骨细胞凋亡

目的：软骨细胞凋亡是骨关节炎重要的发病机制，芒果苷具有抗炎和抗凋亡等多种药理作用，然而芒果 苷对软骨细胞凋亡的作用尚不清楚。本研究探讨芒果苷对IL-1β诱导的软骨细胞凋亡的影响。方法：将小鼠软骨细胞 ATDC5随机分为control组、IL-1β组、MFN-L组、MFN-M组、MFN-H组及MFN+LY294002组。Control组细胞不加 任何干预；IL-1β组细胞用IL-1β(10 ng/mL)处理24 h；MFN-L、MFN-M、MFN-H组细胞先分别用5、10、20 μmol/L 的芒果苷预处理 1 h，再用 IL-1β(10 ng/mL)处理 24 h；MFN+LY294002 组细胞先加入 LY294002(25 μmol/L)处理 1 h， 再加入芒果苷(20 μmol/L)处理1 h，最后再用IL-1β(10 ng/mL)处理24 h。用CCK-8检测细胞活力，流式细胞术检测细 胞凋亡，比色法检测caspase-3活性，蛋白质印迹法检测Bcl-2，Bax及磷脂酰肌醇-3-激酶/蛋白激酶B(PI3K/Akt)通路 相关蛋白质的表达。结果：与control组相比，IL-1β组细胞活力显著降低，细胞凋亡率显著上升，caspase-3活性显著 增加，Bax蛋白质表达水平显著上调，Bcl-2、p-PI3K和p-Akt蛋白质表达水平显著下调(均P<0.05)。与IL-1β组相比， MFN-L、MFN-M、MFN-H组细胞活力显著升高，细胞凋亡率显著下降，caspase-3活性显著下降，Bax蛋白质表达水 平显著下调，Bcl-2、p-PI3K和p-Akt蛋白质表达水平显著上调(均P<0.05)。与MFN-M组相比，MFN+LY294002组细 胞凋亡率显著上升，Bax蛋白质表达水平显著上调，Bcl-2蛋白质表达水平显著下调(均P<0.05)。结论：芒果苷可减 轻IL-1β诱导的软骨细胞凋亡，其保护作用是通过激活PI3K/Akt通路实现的。

Mangiferin attenuates IL-1β-induced chondrocytes apoptosis

Objective: Chondrocyte apoptosis is an important process in the pathogenesis of osteoarthritis. Mangiferin exerts multiple pharmacological effects such as anti inflammatory and anti-apoptosis. However, the role of mangiferin in chondrocyte apoptosis is not clear. In this study, we aimed to explore the role of mangiferin in IL-1β -induced chondrocyte apoptosis. Methods: ATDC5 cells were randomly divided into a control group, a IL-1β group, a MFN L group, a MFN-M group, a MFN-H group and a MFN+LY294002 group. Cells in the control group were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN-L group, the MFN-M group and the MFN-H group were pretreated with 5, 10 and 20 μmol/L mangiferin for 1 h respectively, and then they were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN+LY294002 group were treated with LY294002 (25 μmol/L) for 1 h, then mangiferin (20 μmol/L) and IL-1β (10 ng/mL) for 1 h and 24 h, respectively. Cell viability was detected by CCK-8 assay and cell apoptosis was measured by flow cytometry. Colorimetric assay was conducted to measure the caspase-3 activity. The protein levels of Bcl-2, Bax, and phosphoinositide 3-kinase (PI3K)/Akt signaling pathway related proteins were detected by Western blotting. Results: Compared to the control group, cell viability was significantly decreased; cell apoptosis, caspase-3 activity and Bax protein expression were significantly increased; the protein levels of Bcl-2, p-PI3K, and p-Akt were significantly decreased in the IL-1β group (all P<0.05). Compared to the IL-1β group, cell viability was significantly increased; cell apoptosis, caspase-3 activity and Bax protein expression were significantly decreased; the protein levels of Bcl-2, p-PI3K, and p-Akt were significantly increased in the MFN-L, the MFN-M and the MFN-H groups (all P<0.05). Compared to the MFN-M group, cell apoptosis and the protein level of Bax in the MFN+LY294002 group were significantly increased; the Bcl-2 protein expression was significantly decreased (all P<0.05). Conclusion: Mangiferin could attenuate IL-1β-induced apoptosis of the mice chondrocytes, which is mediated by the activation of PI3K/Akt signaling pathway.