GC-MS法分析黄皮叶和假黄皮叶中挥发油成分的差异

目的:分析黄皮叶和假黄皮叶中挥发油成分的差异。方法:通过水蒸气蒸馏法分别提取黄皮叶和假黄皮叶中的挥发油。然后采用气相色谱-质谱联用(GC-MS)技术进样分析获得总离子流图,采用HPMSD化学工作站对总离子流图中各色谱峰进行质谱扫描后,通过检索比对图谱库NIST Version 1.7鉴定两种药材中挥发油的化学成分,并采用峰面积归一化法计算各成分的相对质量分数。结果:从黄皮叶、假黄皮叶挥发油中分别鉴定出了43、31个成分,相对质量分数总和分别为97.59%、98.57%。其中,相对质量分数超过1%的成分分别有19、18个,均主要为倍半萜类;相对质量分数超过5%的挥发油成分分别有7、5个,在黄皮叶挥发油中主要以(-)-斯巴醇(12.35%)和(E)-5-{(1R,3R,6S)-2,3-二甲基三环2.2.1.02,6]庚烷-3-基}-2-甲基戊-2-烯醛(14.70%)为主,在假黄皮叶中主要以(E)-倍半水合桧烯(24.94%)和1-(1,5-二甲基-4-己烯基)-4-甲基-苯(16.15%)为主。两者中共有挥发油成分4个,分别为α-蛇麻烯、(E)-5-{(1R,3R,6S)-2,3-二甲基三环2.2.1.02,6]庚烷-3-基}-2-甲基戊-2-烯醛、石竹烯氧化物和(-)-斯巴醇,两者中共有成分含量差异均不大。结论:黄皮叶和假黄皮叶挥发油中成分类型基本相似,但具体成分组成和含量差异较大,不可相互替代使用。

Analysis of the Difference of Volatile Oil Components from the Leaves of Clausena lansium and Clausena excavata by GC-MS

OBJECTIVE:To analyze the difference of volatile oil components from the leaves of Clausena lansium and Clausena excavata.METHODS:The volatile oil was extracted from the leaves of C.lansium and C.excavata by steam distillation.GC-MS method was adopted to analyze volatile oil to obtain TIC.After mass spectra scanning of the chromatographic peaks in the TIC diagram by HPMSD chemical workstation,chemical components of volatile oil in 2 kinds of samples were identified by retrieving and comparing mass spectrum database NIST Version 1.7.The peak area normalization method was used to calculate the relative mass fraction of each component.RESULTS:A total of 43 and 31 kinds of components were identified in volatile oil from the leaves of C.lansium and C.excavata;total relative mass fractions were 97.59%and 98.57%.Relative mass fractions of 19 and 18 components in volatile oil from the leaves of C.lansium and C.excavata were more than 1%,mainly being sesquiterpenoids.Relative mass fractions of 7 and 5 components in volatile oil from the leaves of C.lansium and C.excavata were more than 5%;the volatile components in volatile oil from the leaves of C.lansium were mainly(-)-spatol(12.35%)and(E)-5-{(1R,3R,6S)-2,3-dimethyltricyclic2.2.1.02,6]heptane-3-yl}-2-methyl pentane-2-enol(14.70%);those from the leaves of C.excavata were mainly(E)-sesquihydrated betuline(24.94%)and 1-(1,5-dimethy-4-hexenyl)-4-methyl-benzene(16.15%).A total of 4 components were found in volatile oil from the leaves of C.lansium and C.excavata,mainly beingα-humulene,(E)-5-{(1R,3R,6S)-2,3-dimethyltricyclic2.2.1.02,6]heptane-3-yl}-2-methylpentaeryl-2-enol,caryophyllene oxide and(-)-spatol;the content differences of them were not significant.CONCLUSIONS:The components of volatile oils from the leaves of C.lansium and C.excavata are basically similar However,the composition and comtent of specific components are quiet different and can not substituted for each other.