Quantification of granulocyte-macrophage colony-stimulating factor hypersensitivity in juvenile myelomonocytic leukemia by 3H-thymidine assay |
| |
Authors: | Tanaka Makito Takahashi Yoshiyuki Xu Yinyan Yoshida Nao Yoshimi Ayami Villalobos Itzel Bustos Hama Asahito Nishio Nobuhiro Hidaka Hirokazu Wang Yue Yagasaki Hiroshi Kojima Seiji |
| |
Affiliation: | Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. |
| |
Abstract: | Recent molecular studies have revealed that the GM-CSF/RAS signaling pathway plays a central role in the pathogenesis of juvenile myelomonocytic leukemia (JMML). CFU-GM colony assay is an important test for GM-CSF hypersensitivity in patients with JMML, but requires specific skills. We established a simple and easy quantification method to test GM-CSF hypersensitivity, using a (3)H-thymidine assay. With this quantification method, JMML patients with RAS mutations showed significantly higher GM-CSF sensitivity than JMML patients with PTPN11 mutations. This method will be useful not only in the diagnosis of JMML, but also to evaluate the difference of GM-CSF sensitivity among patients. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|