Sequencing of avian influenza virus genomes following random amplification |
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Authors: | Afonso Claudio L |
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Affiliation: | U.S. Department of Agriculture, Athens, CA, USA. cafonso@seprl.usda.gov |
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Abstract: | Increasing surveillance for the avian influenza virus (AIV) has underscored the need for quickly and precisely characterizing isolates of this highly variable target. Random amplification, sequencing, and assembly of total RNA from nonpurified virus overcomes the need for specific primers for DNA microarray or PCR protocols. |
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