| 脂质体转染AFP基因构建树突状细胞肝癌瘤苗及体外活性检测 |
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| 引用本文： | 范强,钟翠平,陈空,王国强,傅继东,吴超群.脂质体转染AFP基因构建树突状细胞肝癌瘤苗及体外活性检测[J].中国免疫学杂志,2002,18(4):255-258. |
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| 作者姓名： | 范强 钟翠平 陈空 王国强 傅继东 吴超群 |
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| 作者单位： | 1. 复旦大学医学院组织胚胎学教研室,上海,200032
2. 复旦大学遗传所,上海,200032 |
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| 基金项目： | 国家自然科学基金（No．39770387），高校博士点基金 （No．9839）资助 |
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| 摘 要： | 目的：探讨以ＡＦＰ为靶点，构建ＡＦＰ－ＤＣ肝癌瘤苗及其抗肝癌免疫治疗的可能性。方法：应用脂质体将ＡＦＰ基因转染体外培养的未成熟 ＢＭＤＣ，构建 ＡＦＰ－ＤＣ肝癌瘤苗，以流式细胞术、Ｗｅｓｔｅｒｎ ｂｌｏｔ、３Ｈ－ＴｄＲ法和 ＭＴＴ法等检测其免疫活性。结果：ＡＦＰ－ＤＣ瘤苗不仅能产生和分泌ＡＦＰ，而且能上调自身的Ｂ７分子和ＭＨＣ分子，明显刺激Ｔ细胞增殖及提高ＣＴＬ的杀伤作用。结论：提示肝癌相关基因ＡＦＰ可作为抗肝癌基因治疗的切入点。
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| 关 键 词： | 树突状细胞 DNA瘤苗 AFP 基因传染 |
| 文章编号： | 1000-484X(2002)04-0255-04 |
The construction of dendritic cell-based hepatocellular carcinoma vaccine encording AFP |
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| FAN Qiang,ZHONG Cui-Ping,CHEN Kong et al.The construction of dendritic cell-based hepatocellular carcinoma vaccine encording AFP[J].Chinese Journal of Immunology,2002,18(4):255-258. |
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| Authors: | FAN Qiang ZHONG Cui-Ping CHEN Kong |
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| Affiliation: | FAN Qiang,ZHONG Cui-Ping,CHEN Kong et al. Department of Histology and Embryology,Medical Center of Fudan University . Genetics Institute of Fudan University,Shanghai 200032 |
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| Abstract: | Objective: To construct an AFP-DC vaccine and investigate its therapeutic function against HCC Methods: AFP was successfully transfected into cultured immature BMDC with lipofectin. Antitumor activity of the vaccine was studied in vitro by using Western blot, flow cytometry, MLR and cototoxicty assay. Results: The vaccine was confirmed capable of not only synthesizing and secreting AFP, but also up-regulating the expression of surface molecules of B7 and MI-IC. The immunization was effective in priming of specific responses and immune protection against HCC. Conclusion:The results indicated that AFP could be considered as a traget in gene immunotherapy of HCC. |
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| Keywords: | Dendritic cell DNA vaccine AFP Transfection |
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