|
|||||
|
|
| 2006～2007年流行流感病毒减毒疫苗株的构建 | |
| 引用本文： | 杨鹏辉,叶艺,石辛甫,罗德炎,张钰,颜艳,张鹏飞,段跃强,邢丽,刘秀梵,王希良.2006～2007年流行流感病毒减毒疫苗株的构建[J].生物化学与生物物理进展,2008,35(3):312-319. |
| 作者姓名： | 杨鹏辉 叶艺 石辛甫 罗德炎 张钰 颜艳 张鹏飞 段跃强 邢丽 刘秀梵 王希良 |
| 作者单位： | 1. 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071 2. 西北农林科技大学动物科技学院,杨陵,712100 3. 扬州大学,农业部畜禽传染病学重点开放实验室,扬州,225009 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 选择冷适应、温度敏感、减毒的A/Ann Arbor/6/60(H2N2)流感病毒株作为骨架病毒,对其6个内部基因片段进行了全基因合成,同时人工引入5个氨基酸突变(PB1-391E,581G,661T,PB2-265S,NP-34G).HA和NA来源于2006-2007当年流行株A/New Caledonia/20/99(H1N1).8个基因片段通过与改造后的转录载体pAD3000连接,构建8个基因的拯救载体,经测序获得序列准确的拯救质粒:pMDV-A-PB2、pMDV-A-PB1、pMDV-A-PA、pMDV-A-NP、pMDV-A-M、pMDV-A-NS、pMDV-A-HA、pMDV-A-NA.6质粒与当年流行株的表面基因HA和NA进行"6 2"组合的病毒拯救,8个重组质粒共转染COS-1细胞,成功拯救出了具有血凝性的冷适应减毒的重组A型人流感病毒.鸡胚尿囊液中重组病毒的血凝效价为l:279-1:210.构建的A/AA/6/60 6个内部基因的病毒骨架拯救系统,为深入研究冷适应减毒人流感病毒的基因功能和新型疫苗研发奠定了基础. |
| 关 键 词： | 流感病毒 反向遗传学 8质粒拯救系统 重组流感病毒 influenza virus reverse genetics eight-plasmid system reassorted influenza virus 流感 病毒 毒疫苗株 Vaccines Influenza Virus human system genes used research function vaccine candidate chicken eggs influenza A virus results containing cells internal |
| 收稿时间： | 2007/7/16 0:00:00 |
| 修稿时间： | 2007年7月16日 |
Construction of Attenuated Influenza Virus Vaccines of Vaccines of-2006-2007 |
|
| YANG Peng-Hui,YE Yi,SHI Xin-Fu,LUO De-Yan,ZHANG Yu,YAN Yan,ZHANG Peng-Fei,DUAN Yue-Qiang,XING Li,LIU Xiu-Fan and WANG Xi-Liang.Construction of Attenuated Influenza Virus Vaccines of Vaccines of-2006-2007[J].Progress In Biochemistry and Biophysics,2008,35(3):312-319. | |
| Authors: | YANG Peng-Hui YE Yi SHI Xin-Fu LUO De-Yan ZHANG Yu YAN Yan ZHANG Peng-Fei DUAN Yue-Qiang XING Li LIU Xiu-Fan and WANG Xi-Liang |
| Affiliation: | Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;College of Animal Science and Technology, Northwest Sci & Tech University of Agriculture and Forestry, Yangling 712100, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China;Key Laboratory for Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University,Yangzhou 225009, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China |
| Abstract: | |
| Keywords: | influenza virus reverse genetics eight-plasmid system reassorted influenza virus |
| 本文献已被 维普 万方数据 等数据库收录！ | |
| 点击此处可从《生物化学与生物物理进展》浏览原始摘要信息 | |
| 点击此处可从《生物化学与生物物理进展》下载全文 | |
