Growth of <Emphasis Type="Italic">Trametes versicolor</Emphasis> on phenol |
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Authors: | H Yemendzhiev M Gerginova A Krastanov I Stoilova Z Alexieva |
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Affiliation: | (1) The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bontchev Str., bl. 26, 1113 Sofia, Bulgaria;(2) Department of Biotechnology, University of Food Technologies, Maritza blvd., 26, 4002 Plovdiv, Bulgaria |
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Abstract: | Trametes versicolor 1 was shown to grow on phenol as its sole carbon and energy source. The culture growth and degradation ability dependence
on culture medium pH value was observed. The optimal pH value of a liquid Czapek salt medium was 6.5. The investigated strain
utilized completely 0.5 g/l phenol in 6 days. The dynamics of the phenol degradation process was investigated. The process
was characterized by specific growth rate μmax 0.33 h−1, metabolic coefficient k = 4.4, yield coefficient Y
x/s
= 0.23 and rate of degradation Q = 0.506 h−1. The intracellular activities of phenol hydroxylase (0.333 U/mg protein) and cis,cis-muconate lactonizing enzyme (0.41 U/mg protein) were demonstrated for the first time in this fungus. In an attempt to estimate
the occurrence of gene sequences in T. versicolor 1 related to phenol degradation pathway a dot blot analysis with total DNA isolated from this strain was performed. Two synthetic
oligonucleotides were used as hybridizing probes. One of the probes was homologous to the 5′end of phyA gene coding for phenol hydroxylase in Trichosporon cutaneum ATCC 46490. The other probe was created on the basis of cis,cis-muconate lactonizing enzyme coding gene in T. cutaneum ATCC 58094. The results of these investigations showed that T. versicolor 1 may carry genes similar to those of Trichosporon cutaneum capable to degrade phenol. |
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Keywords: | Trametes versicolor Phenol biodegradation Phenol hydroxylase Cis cis-muconate lactonizing enzyme Dot blot analysis |
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