| 单个胎儿有核红细胞的DNA分析 |
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| 引用本文： | 邹丽,朱剑文,张会军,李旻,李敏.单个胎儿有核红细胞的DNA分析[J].临床血液学杂志,2000,13(4):175-176. |
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| 作者姓名： | 邹丽 朱剑文 张会军 李旻 李敏 |
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| 作者单位： | 同济医科大学附属协和医院妇产科!武汉，430022，同济医科大学附属协和医院妇产科!武汉，430022，同济医科大学附属协和医院妇产科!武汉，430022，同济医科大学附属协和医院妇产科!武汉，430022，同济医科大学附属协和医院妇产科!武汉，430022 |
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| 基金项目： | 国家教委留学回国人员启动基金!资助项目 (No.96 6 44 )，卫生部回国人员启动基金!资助项目 (No. 96 0 2 412 ) |
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| 摘 要： | 目的：获取孕妇外周血中的极少量胎儿有核红细胞（ＮＲＢＣ），并进行单个ＮＲＢＣ的基因分析，探讨其在产前基因诊断中的价值。方法：将孕妇外周血进行不连续密度梯度离心，将分离后的细胞进行制片，光学显微镜下识别ＮＲＢＣ，然后用显微操作仪获取单个ＮＲＢＣ进行引物延伸预扩增（ＰＥＰ）及Ｙ染色体特异性ＤＹＺ１基因的聚合酶链反应（ＰＣＲ），以确定其来源于胎儿。结果：单个ＮＲＢＣ的ＰＥＰ－ＰＣＲ法检测结果，６０％检出
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| 关 键 词： | 妥妇外周血 单个胎儿有核红细胞 产前诊断 |
Genetic analysis of single fetal nucleated red blood cell from maternal blood |
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| ZOU Li,ZHU Jian-wen,ZHANG Hui-jun,LI-Min,LI-Min.Genetic analysis of single fetal nucleated red blood cell from maternal blood[J].Journal of Clinical Hematology,2000,13(4):175-176. |
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| Authors: | ZOU Li ZHU Jian-wen ZHANG Hui-jun LI-Min LI-Min |
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| Abstract: | Objective:To study the feasibility of usintg single fetal nucleated red blood cell(NRBC) for prenatal genetic analysis.Method:Fetal cells were isolated from maternal blood with discontinuous density gradient centrifugation.Single NRBC was found and retrieved using a micromanipulator under a microscope.To determine whether the origin of the NRBC was maternal of fetal,the single NRBC was analysed by primer extension preamplification(PEP)and polymerase chain reaction(PCR)to determine the presence of a Y chromosome specific repeat sequence.Result:We have determined the presence of DYZ 1 specific repeat sequence by single NRBC. The male pregnancy was correctly predicted in 60%.Conclusion:Using single fetal NRBC for prenatal genetic analysis is feasible. |
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| Keywords: | Maternal peripheral blood Single fetal nucleated red blood cell Primer extension preamplification Prenatal diagnosis |
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