| PI3K/Akt抑制剂LY294002对胃癌细胞化疗增敏作用的探讨 |
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| 引用本文： | 张晔,曲秀娟,刘云鹏,杨向红,侯科佐,滕月娥,张敬东.PI3K/Akt抑制剂LY294002对胃癌细胞化疗增敏作用的探讨[J].山东医药,2008,48(41):10-11. |
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| 作者姓名： | 张晔 曲秀娟 刘云鹏 杨向红 侯科佐 滕月娥 张敬东 |
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| 作者单位： | 1. 中国医科大学附属第一医院,辽宁沈阳,110001
2. 中国医科大学附属盛京医院 |
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| 基金项目： | 国家自然科学基金
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辽宁省科技攻关计划
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| 摘 要： | 目的 探讨PI3K/Akt特异性抑制剂LY294002与化疗药物5-Fu及奥沙利铂联合使用对3种胃癌细胞系(MGC803、BGC823和SGC7901)化疗效果的影响.方法 将PI3K/Akt特异性抑制剂LY294002联合化疗药物5-Fu及奥沙利铂作用于3种胃癌细胞系,MTT法检测单独使用5-Fu、奥沙利铂及联合LY294002对体外培养的3种胃癌细胞系的增殖抑制作用,流式细胞术检测细胞凋亡.结果 联合LY294002作用后,5-Fu、奥沙利铂对3种胃癌细胞系的增殖抑制作用明显增强(P<0.05),且凋亡率显著提高(P<0.05).结论结果 LY294002能有效提高化疗药物5-Fu、奥沙利铂体外对胃癌细胞的增殖抑制作用,抑制PI3K/Akt信号转导通路可显著提高胃癌的化疗疗效.
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| 关 键 词： | PI3K/Akt信号通路 胃肿瘤 细胞凋亡 |
Effect of PI3K/Akt inhibitor LY294002 on chemotherapeutic sensitivity in human gastric cancer |
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| ZHANG Ye,QU Xiu-juan,LIU Yun-peng,YANG Xiang-hong,HOU Ke-zuo,TENG Yue-E,ZHANG Jing-dong.Effect of PI3K/Akt inhibitor LY294002 on chemotherapeutic sensitivity in human gastric cancer[J].Shandong Medical Journal,2008,48(41):10-11. |
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| Authors: | ZHANG Ye QU Xiu-juan LIU Yun-peng YANG Xiang-hong HOU Ke-zuo TENG Yue-E ZHANG Jing-dong |
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| Affiliation: | 1 The First Hospital;China Medical University;Shenyang 110001;P.R.China |
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| Abstract: | Objective To explore the effect of PI3K/Akt inhibitor LY294002 on the sensitivities of chemotherapeutic agents(5-Fu and Oxaliplatin) in human gastric cancer MGC803,BGC823 and SGC7901 cell lines.Methods MGC803,BGC823 and SGC7901 cells were treated with LY294002.The drug sensitivity was detected by MTT method.The apoptosis ratio was detected using flow cytometer(FCM).Results The inhibition ratios of proliferation of gastric cancer cells were significantly increased by 5-Fu,Oxaliplatin in combined with LY29400... |
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| Keywords: | PI3K/Akt signaling pathway gastric cancer apoptosis |
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