组织蛋白酶G在光老化皮肤成纤维细胞中的表达及意义

目的 探讨组织蛋白酶G（cathepsin G）在光老化皮肤中的表达变化及意义.方法 培养原代人皮肤成纤维细胞,8-甲氧补骨脂素＋长波紫外线（8-MOP＋UVA）法体外诱导培养细胞光老化.衰老相关-β半乳糖苷酶（SA-β-Gal）染色证明老化诱导成功.蛋白印迹技术及反转录-聚合酶链式反应（RT-PCR）对比检测光老化成纤维细胞及正常成纤维细胞组织蛋白酶G蛋白及基因表达.结果 Western印迹法结果 示光老化诱导组组织蛋白酶G表达上调为单UVA诱导组、单8-MOP孵育组及空白组的（1.70±0.028）倍.实时RT-PCR结果 示光老化细胞组织蛋白酶G mRNA表达上调为空白组的（1.42±0.09）倍（P〈0.01）.结论 组织蛋白酶G在光老化成纤维细胞中高表达,可能通过改变细胞外基质成分和激活基质金属蛋白酶参与皮肤光老化所致.

Expression of cathepsin G in photoaged fibroblasts

Objective To investigate the role of cathepsin G in photoaged fibroblasts. Methods Human fibroblasts were cultured and induced to premature senescence using UVA + MOP methods. Senescence-associated-β-galactosidase (SA-β-gal) stain was used to evaluate the positive rate of aged cells. The mRNA and protein expression of cathepsin G in photoaged fibroblasts were detected by real-time RT-PCR and Western blot techniques. Results Over 98 % induced cells presented a positive SA-β-gal straining. The expression of cathepsin G, detected by Western blot, was increased to (1. 70±0. 028) times of the control. And RT-PCR revealed that the synthesis of cathepsin G mRNA was also up-regulated to 1. 42±0. 09. Conclusion The results of our study demonstrates a significant correlation between photoaged fibroblasts and cathepsin G. The up-regulation of cathepsin G may play an important role in the damages of extracellular matrix and activation of MMPS in photoaged human skin.