| 维生素A酸对小鼠囊胚Fas蛋白表达的影响 |
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| 引用本文： | 蔡惠兰,张端莲,熊彦娥,陈晓娇,杨勇.维生素A酸对小鼠囊胚Fas蛋白表达的影响[J].武汉大学学报(医学版),2005,26(6):734-736,751,i0001. |
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| 作者姓名： | 蔡惠兰 张端莲 熊彦娥 陈晓娇 杨勇 |
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| 作者单位： | 1. 咸宁医学院妇产科学教研室,咸宁,437100
2. 武汉大学医学院人体解剖与组织胚胎学系,武汉,430071 |
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| 摘 要： | 目的:探讨全反式维生素A酸(ATRA)对体外培养小鼠囊胚Fas蛋白表达的作用,并了解其对着床前期胚胎发育的作用。方法:获取妊娠3.5 d小鼠囊胚,分别培养在含0、1μmol.L-1和10μmol.L-1的ATRA的M199培养基中24 h,用免疫组织化学SP法,分别检测不同剂量组ATRA对小鼠囊胚Fas蛋白表达的状况。结果:ATRA可增强小鼠囊胚Fas蛋白表达,表达强度呈现剂量依赖型。结论:ATRA对小鼠胚胎的发育具有细胞毒性作用,Fas在小鼠胚胎发育的调节中发挥了重要的作用。
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| 关 键 词： | Fas 全反式维生素A酸 囊胚 小鼠 |
| 文章编号： | 1671-8852（2005）06-0734-03 |
| 收稿时间： | 2005-05-08 |
| 修稿时间： | 2005-05-08 |
Effect of Retinoic Acid on the Expression of Fas Proteins in Mouse Blastocysts |
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| Cai Huilan,Zhang Duanlian,Xiong Yan'e,et alDept.of Obstetrics and Gynecology,Xianning Medical School,Xianning ,China.Effect of Retinoic Acid on the Expression of Fas Proteins in Mouse Blastocysts[J].Medical Journal of Wuhan University,2005,26(6):734-736,751,i0001. |
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| Authors: | Cai Huilan Zhang Duanlian Xiong Yan'e Deptof Obstetrics and Gynecology Xianning Medical School Xianning China |
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| Affiliation: | Cai Huilan,Zhang Duanlian,Xiong Yan'e,et alDept.of Obstetrics and Gynecology,Xianning Medical School,Xianning 437100,China |
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| Abstract: | Objective: To study the effect of all-trans retinoic acid(ATRA) on the expression of Fas protein in mouse blastocysts in vitro and its possible roles in preimplantation mouse embryo development.Methods: Mouse 3.5 d blastocysts were grown in doses of 0,1 and 10 μmol·L~(-1) all-trans retinoic acid for 24 h and observed for their expression of Fas proteins by immunohistochemical SP staining.Results: The expression of Fas protein affected by ATRT in mouse blastocysts was dose-dependent.Conclusion: This study examined the cytotoxic effect of ATRA on mouse embryos.Fas may play critical regulating roles in mouse embryo development. |
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| Keywords: | Fas All-trans Retinoic Acid Blastocyst Mouse |
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