薯蓣粥对2型糖尿病大鼠肠道通透性的影响

目的:探讨薯蓣粥对2型糖尿病（Ttype 2 diabetes mellitus，T2DM）大鼠肠道通透性的影响。方法:将35只雄性Wistar随机分为空白组8只和造模组27只。空白组大鼠饲喂普通饲料，造模组使用高脂喂养加腹部注射链脲佐菌素法制备T2DM大鼠模型，成模后大鼠随机分为模型组、薯蓣粥组、二甲双胍组；空白组、模型组每日以生理盐水灌胃5 mL，薯蓣粥组每日以薯蓣粥灌胃5 mL，二甲双胍组每日以二甲双胍水溶液灌胃5 mL，共6周；每周检测大鼠空腹血糖（Fasting Blood Glucose，FBG），干预后观察各组大鼠结肠组织学形态，检测结肠组织的炎性因子肿瘤坏死因子-α（Tumor necrosis factor-α，TNF-α）、白介素-6（Interleukin-6，IL-6），和紧密连接蛋白Occludin、ZO-1的表达；以及血清连蛋白（Zonulin）、内毒素（Lipopolysaccharide，LPS）水平；血清TNF-α、IL-6、空腹胰岛素（Fasting insulin，FINS）水平，计算胰岛素抵抗指数（HOMA-IR）。结果:模型组大鼠的肠绒毛断裂不全且间隔稀疏，同时伴有中性粒细胞渗出，薯蓣粥组、二甲双胍组大鼠的上述情况优于模型组。与空白组相比，模型组大鼠结肠TNF-α、IL-6蛋白表达增加（P<0.05），Occludin、ZO-1蛋白表达减少（P<0.05），血清中Zonulin、LPS、TNF-α、IL-6、FINS水平上升（P<0.05），FBG、HOMA-IR升高（P<0.05）；与模型组相比，薯蓣粥组大鼠结肠TNF-α、IL-6蛋白表达减少（P<0.05），Occludin、ZO-1蛋白表达增加（P<0.05）；血清中Zonulin、LPS、TNF-α、IL-6、FINS水平下降（P<0.05），FBG、HOMA-IR下降（P<0.05）。结论:薯蓣粥能够降低T2DM大鼠肠道通透性，进而达到减轻全身炎症反应，缓解胰岛素抵抗、改善血糖代谢的效果。

Effect of Yam Gruel on Intestinal Permeability of Type 2 Diabetic Rats

Objective:To explore the effect of yam gruel on intestinal permeability in T2DM rats.Methods:35 male Wistar rats were randomly divided into blank group(n=8)and model group(n=27).The rats in the blank group were given normal diet,and the model group were fed with high-fat diet and intraperitoneal injection of streptozotocin to prepare T2DM rat model.After modeling,the rats were randomly divided into model group,yam gruel group and metformin group.The blank group and model group were given 5 mL normal saline,the yam gruel group was given 5 mL yam gruel,and the metformin group was given 5 mL metformin.Fasting blood glucose(FBG)was measured every week.After the intervention,the histological morphology of colon was observed,and the protein expressions of TNF-α,IL-6,Occludin and ZO-1 in colon tissue,and the expression of Zonulin,LPS,TNF-α,IL-6 and fins in serum were detected.HOMA-IR was calculated.Results:In the model group,the intestinal villi were incomplete and sparsely spaced,accompanied by neutrophil exudation.The above situation in yam gruel group and metformin group was better than that in the model group.In the model group,the protein expression of TNF-αand IL-6 increased(P<0.05),the protein expression of Occludin and ZO-1 decreased(P<0.05),the expression of Zonulin,LPS,TNF-α,IL-6 and FINS increased(P<0.05),FBG and HOMA-IR increased(P<0.05).In the yam gruel group,the protein expression of TNF-αand IL-6 decreased(P<0.05),the protein expression of Occludin and ZO-1 increased(P<0.05),the expression of Zonulin,LPS,TNF-α,IL-6 and FINS decreased(P<0.05),FBG and HOMA-IR decreased(P<0.05).Conclusion:Yam gruel could reduce the intestinal permeability of T2DM rats,and then reduce the systemic inflammatory response,alleviate insulin resistance and improve blood glucose.