| 用于放射性砹(碘)标记蛋白质的偶联剂SPC的合成及表征 |
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| 引用本文： | 唐军,金建南,刘宁,廖家莉,杨远友.用于放射性砹(碘)标记蛋白质的偶联剂SPC的合成及表征[J].原子能科学技术,2004,38(3):261-265. |
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| 作者姓名： | 唐军 金建南 刘宁 廖家莉 杨远友 |
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| 作者单位： | 四川大学,原子核科学技术研究所,辐射物理及技术教育部重点实验室,四川,成都,610064 |
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| 基金项目： | 国家自然科学基金(29601104,20171033)，高等学校博士学科点专项科研基金(2003610016) |
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| 摘 要： | 以5-溴烟酸为起始物，通过3步主要反应合成了一种适用于蛋白质放射性砹(碘)标记的偶联剂——5-(三正丁基锡)-3-吡啶甲酸N-琥珀酰亚胺酯(SPC)，并用核磁共振(NMR)、质谱(MS)、高效液相色谱(HPLC)等进行了表征。以该试剂为双功能偶联剂，实现了IgG的^125I标记，标记率达30％以上。标记产物在体外具有较高的稳定性。
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| 关 键 词： | 偶联剂 放射性砹 合成 表征 蛋白质标记 放射性碘 |
| 文章编号： | 1000-6931(2004)03-0261-05 |
Synthesis and Characterization of SPC as a Linker for Labeling Proteins With Astatine and Iodine Radioisotopes |
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| TANG Jun,JIN Jian-nan,LIU Ning,LIAO Jia-li,YANG Yuan-you Key Laboratory for Radiation Physics and Technology of Education Ministry of China.Synthesis and Characterization of SPC as a Linker for Labeling Proteins With Astatine and Iodine Radioisotopes[J].Atomic Energy Science and Technology,2004,38(3):261-265. |
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| Authors: | TANG Jun JIN Jian-nan LIU Ning LIAO Jia-li YANG Yuan-you Key Laboratory for Radiation Physics and Technology of Education Ministry of China |
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| Affiliation: | TANG Jun,JIN Jian-nan,LIU Ning,LIAO Jia-li,YANG Yuan-you Key Laboratory for Radiation Physics and Technology of Education Ministry of China,Institute of Nuclear Science and Technology,Sichuan University,Chengdu 610064,China |
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| Abstract: | N-succinimidyl 5--(tributylstannyl)-3-pyridinecarboxylate (SPC), a useful linker for
labeling proteins with astatine and iodine radioisotopes, is synthesized in three steps with 5-
bromonicotinic acid as a starting material, and is characterized by nuclear magnetic resonance
(NMR), mass spectrometer(MS) and high performance liquid chromatography (HPLC). With
this bi-functional ester, ~(125)I labeled human IgG is performed in labeling yield of approximately
30%. Also, the in vitro stability of the prepared ~(125)I-IgG is evaluated. |
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| Keywords: | linker synthesis characterization ~(211)At ~(125)I labeling |
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