MMP-2在食管癌中的表达及其与血管生成的相关性

目的观察食管癌组织、癌旁中重度不典型增生组织和切缘正常组织中基质金属蛋白酶．2(MMP-2)的表达及其与微血管密度(MVD)的关系,探讨。MMP-2对食管癌血管生成的作用和意义。方法应用SP法对48例食管癌组织、17例癌旁中重度不典型增生组织和12例切缘正常组织进行MMP-2和CD34免疫组化染色,检测癌组织、癌旁不典型增生组织和正常黏膜组织中MMP-2的表达和MVD,并分析MMP-2的表达与：MVD之间,以及它们与食管癌临床病理特征之间的关系。结果食管癌组织中MMP-2的表达显著高于癌旁组织,癌旁组织MMP-2的表达亦明显高于切缘正常组织。食管癌和癌旁组织中MMP-2的表达,与肿瘤内及癌旁组织内的平均MVD呈正相关;。MMP-2表达和肿瘤中MVD计数与食管癌的淋巴结转移密切相关。结论MMP-2可能在食管癌的血管生成和侵袭转移中起重要作用,可能是食管癌的重要促血管生成因子之一。

Correlation between expression of matrix metalloproteinase-2 and angiogenesis in esophageal carcinoma

OBJECTIVE: To study expression of MMP-2 in relation to microvessel density (MVD) in esophageal carcinoma. METHODS: Forty-eight specimens of esophageal carcinoma (Ec) and 17 specimens of grade II + III epithelial dysplasia (Dy) close to the tumor and 12 specimens of normal tissue (Nt) along the incisional margin were examined by S-P immunohistochemical staining with anti-MMP-2 monoclonal antibody. An anti-CD34 monoclonal antibody was used to show MVD. RESULTS: MMP-2 expression in Ec was remarkably higher than that in Dy, which was higher than that in Nt. MMP-2 expression in Ec and Dy was significantly correlated with MVD in the tumor and nearby tissue. MMP-2 expression and MVD in Ec significantly associated with lymph node metastasis. CONCLUSION: Expression of MMP-2 plays an important role in angiogenesis and lymph node metastasis of esophageal cancer.