| 大蒜新素对分离大鼠脑细胞内游离Ca^2＋的影响 |
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| 引用本文： | 马晓红,潘鑫鑫.大蒜新素对分离大鼠脑细胞内游离Ca^2＋的影响[J].中国药理学报,1999,20(7):609-612. |
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| 作者姓名： | 马晓红 潘鑫鑫 |
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| 摘 要： | 观察大蒜新素对不同刺激剂所致分离大鼠脑细胞内游离钙的影响。方法：以Ｆｕｒａ２－ＡＭ为细胞内游离钙的荧光指示剂，用ＡＲ－ＣＭ－ＭＩＣ阳离子测定系统，直接测定了分离新生大鼠脑细胞内游离钙值，观察了大蒜新素的影响。结果；大蒜新素对脑细胞静息「Ｃａ＾２＋」ｉ无明显影响，大蒜新素１－１００μｍｏｌ．Ｌ＾－１能剂量依赖性地抑制高Ｋ＾＋和谷氨酸引起的「Ｃａ＾２＋」ｉ升高，其中ＩＣ５０分别为５９．７和６９．９μｍ
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| 关 键 词： | 大蒜新素 脑细胞 细胞内钙 Fura－2 抗脑缺血 |
Effects of allitridi on intracellular Ca2+ concentration in isolated rat brain cells. |
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| X H Ma,X X Pan,T P Liu.Effects of allitridi on intracellular Ca2+ concentration in isolated rat brain cells.[J].Acta Pharmacologica Sinica,1999,20(7):609-612. |
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| Authors: | X H Ma X X Pan T P Liu |
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| Affiliation: | Department of Pharmacology, Nanjing Medical University, China. |
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| Abstract: | AIM: To study actions of allitridi extracted from garlic on intracellular calcium in isolated rat brain cells. METHODS: Brain cells were isolated from newborn rat brain with Fura 2-AM measurements of intracellular Ca2+ concentration (Ca2+]i). RESULTS: Allitridi 1-100 mumol.L-1 concentration-dependently blocked increases of Ca2+]i caused by potassium chloride and sodium glutamate (Glu) with IC50 of 59.7 and 69.9 mumol.L-1 respectively. Allitridi 100 mumol.L-1 blocked norepinephrine (Nor)-induced Ca2+]i elevation. CONCLUSION: Allitridi is an effective agent for blocking the Ca2+]i increase caused by potassium chloride, Nor and Glu. |
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