钙通道阻塞剂对大鼠肝细胞钙释放激活的钙电流的影响

目的 研究钙通道拮抗剂对大鼠肝细胞钙释放激活的钙电流（ＩＣＲＡＣ）的影响。方法 应用全细胞膜片箝技术。结果：ＩＣＲＡＣ的电流峰值是（－０．４１±０．０９）ｎＡ（ｎ＝１５）反转电位约为０ｍＶ。维拉帕米（Ｖｅｒ）地尔硫Ｚｕｏ（Ｄｉｌ）和硝苯地平（Ｎｉｆ）显著降低ＩＣＲＡＣ不影响它的反转电位，Ｖｅｒ５μｍｏｌ．Ｌ＾－１的抑制率是４０％±１２％（ｎ＝３），Ｖｅｒ５０μｍｏｌ．Ｌ＾－１使ＩＣＲＡＣ的幅值从（

Effects of calcium channel blockers on calcium release-activated calcium currents in rat hepatocytes.

AIM: To study the influences of calcium channel blockers on calcium release-activated calcium currents (ICRAC) in rat hepatocytes. METHODS: Whole-cell patch-clamp technique was used. RESULTS: The peak amplitude of ICRAC was -0.41 nA +/- 0.09 nA (n = 15), its reversal potential was about 0 mV. Verapamil (Ver), diltiazem (Dil), and nifedipine (Nif) decreased ICRAC strikingly, without affecting its reversal potential. The inhibitory rate of Ver 5 mumol.L-1 was 40% +/- 12% (n = 3), Ver 50 mumol.L-1 reduced the peak amplitude of ICRAC from -0.49 nA +/- 0.12 nA to -0.20 nA +/- 0.09 nA (P < 0.01 vs control, n = 5). The inhibitory rate was 57% +/- 15%. Dil 50 mumol.L-1 and Nif reduced ICRAC from -0.43 nA +/- 0.10 nA to -0.29 nA +/- 0.07 nA (P < 0.01 vs control, n = 5), from -0.32 nA +/- 0.08 nA to -0.27 nA +/- 0.08 nA (P < 0.01 vs control, n = 5). The inhibitory rate was 31% +/- 11%, 19% +/- 7%, respectively. The amplitude of ICRAC was dependent on extracellular Ca2+ concentration. The peak amplitude of ICRAC was -0.21 nA +/- 0.08 nA (n = 3) in Tyrode's solution with Ca2+ 1.8 mmol.L-1 (P < 0.01 vs the peak amplitude of ICRAC in external solution with Ca2+ 10 mmol.L-1). CONCLUSION: The three calcium antagonists inhibited ICRAC effectively and protected hepatocytes from calcium overload via the inhibition of ICRAC.