| 金丝桃苷对分离的新生大鼠脑细胞内游离钙浓度的影响 |
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| 引用本文： | 陈志武,马传庚.金丝桃苷对分离的新生大鼠脑细胞内游离钙浓度的影响[J].中国药理学报,1999,20(1):27-30. |
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| 作者姓名： | 陈志武 马传庚 |
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| 摘 要： |
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| 关 键 词： | 金丝桃苷 再灌注损伤 脑缺血 钙 |
Effects of hyperin on free intracellular calcium in dissociated neonatal rat brain cells. |
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| Z W Chen,C G Ma.Effects of hyperin on free intracellular calcium in dissociated neonatal rat brain cells.[J].Acta Pharmacologica Sinica,1999,20(1):27-30. |
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| Authors: | Z W Chen C G Ma |
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| Affiliation: | Department of Pharmacology, Anhui Medical University, Hefei, China. aydxt@public.ah.hf.cn |
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| Abstract: | AIM: To study the effects of hyperin (Hyp) on free intracellular calcium concentration (Ca2+]i) of brain cells. METHODS: The neonatal rat brain cells were dissociated. Ca2+]i in presence and absence of extracellular high K+, L-glutamic acid (Glu), 5-hydroxytryptamine (5-HT), and norepinephrine (NE) were assayed with Fura 2-AM. RESULTS: The resting Ca2+]i in Hanks' solution (CaCl2 1.3 mmol.L-1) was (208 +/- 12) nmol.L-1 (n = 17). Hyp had no significant effects on the resting Ca2+]i. Hyp 1.0, 4.0, and 16.0 mumol.L-1 markedly inhibited the increase of Ca2+]i evoked by K+ 50 mmol.L-1 in a concentration-dependent manner. Hyp 16.0 mumol.L-1 inhibited the increases of Ca2+]i induced by NE 1, 2, 4, and 8 mumol.L-1. Hyp (16.0 mumol.L-1) also markedly attenuated 5-HT and Glu-induced increase of Ca2+]i. CONCLUSION: Hyp possessed inhibitory effects on influx of Ca2+ in the neonatal rat brain cells. |
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