狼毒药材HPLC指纹图谱研究 |
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引用本文: | 邓德红,胡菊.狼毒药材HPLC指纹图谱研究[J].国际检验医学杂志,2016(4). |
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作者姓名: | 邓德红 胡菊 |
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作者单位: | 1. 湖北省孝感麻风防治中心 ,湖北汉川,431600;2. 湖北工业职业技术学院环境工程系 ,湖北十堰,442000 |
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摘 要: | 目的采用高效液相色谱法(HPLC)建立狼毒药材的指纹图谱。方法 2013年10月至2014年10月,使用Hypersil ODS(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相,梯度洗脱,洗脱流速为1.0mL/min,柱温20℃,检测波长210nm,进样20μL。结果对不同来源15批狼毒药材进行了分析,标定了15个共有峰,15个共有峰相对保留时间相对标准差(RSD)≤3.7%,相对峰面积RSD≤3.6%;各色谱峰分离度较好,相似度较高,15批不同来源狼毒药材中,除了广西南宁药材料相似度为0.858,其他产地药材料相似度均在0.95以上。达到了中药指纹图谱的技术要求。结论该方法简便、准确、重现性好,可用于狼毒指纹图谱的测定及质量控制。
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关 键 词: | 狼毒 高效液相 指纹图谱 质量控制 |
Study on HPLC fingerprint of radix euphorbiae fischerianae |
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Abstract: | Objective To establish fingerprint of Radix Euphorbiae Fischerianae by high performance liquid chromatography (HPLC) .Methods The samples were separated on Hypersil ODS(250 mm × 4 .6 mm ,5 μm) column with gradient mobile phase of acetic-water ,the column temperature was 20 ℃ with the flow rate of 1 .0 mL/min and UV detection wavelength was 210 nm .the sample injection was 20 μL .Results 15 samples of different origin Radix Euphorbiae Fischerianae were detected and each chroma-tographic peak was separated well with conforming to the requirements of fingerprint by calibrating the 15 peaks .15 common peak retention time RSD ,relative peak area RSD were 3 .7% ,3 .6% or less respectively ;The chromatographic peak separation degree was better ,similarity was higher ,15 batches of different sources of stellera medicinal materials ,in addition to the Guangxi nanning medi-cine material similarity was 0 .858 ,other origin medicine material similarity above 0 .95 .Have reached the technical requirements of traditional Chinese medicine (TCM ) fingerprint .Conclusion The method is accurate ,reliable ,and the HPLC fingerprint shows good repeatability ,which can be used for one of the quality control of Radix Euphorbiae Fischerianae . |
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Keywords: | Radix Euphorbiae Fischerianae high performance liquid chromatography fingerprints quality control |
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