纤维粘连蛋白及骨形成蛋白-2、4在牙胚发育中的表达研究

目的 观察纤维粘连蛋白(fibronectin,FN)和骨形成蛋白-2、4(BMP-2、4)在大鼠牙胚发育中的表达,探讨其在成牙本质细胞分化及成熟过程的时空表达关系,为明确牙齿发育的分子调控网络提供理论和实验基础.方法 获取SD大鼠蕾状期和钟状期第一磨牙牙胚,通过免疫组化LsAB法观察FN和BMP-2、4的表达差异及相互关系.结果 FN在蕾状期和钟状期都有表达,蕾状期主要分布在牙上皮、牙间质,周围非牙间质细胞为阴性,钟状期主要分布在成牙本质细胞分化活跃的区域和分化中的内釉上皮细胞.钟状期BMP-2、4的表达较强,主要分布在成牙本质细胞分化活跃的区域. 结论 BMP-2、4和FN在牙齿发育的蕾状期和钟状期均有表达,但表达的阳性分布区不同.

Expressions of Fibronectin and Bone Morphogenetic Protein-2, 4 during Development of Mouse Tooth Germ

Objective The purpose of this study was to investigate the distribution and relationship of fibronectin(FN),bone morphogenetic protein(BMP) 2,4 during development of mouse tooth germ.Methods Rat embryonic first molars were collected from E14(bud stage) and E18(bell stage).The expressions of FN and BMP-2,4 were analyzed with immunohistochemistry.Resluts FN was located in the epithelium and dental mesenchyme on bud stage,but on bell stage,the FN was found at the region of differentiating odontoblasts and in the inner enamel epithelium,and also BMP-2,4 were abundant mainly at the brisk region of differentiating odontoblasts.Conclusion The results demonstrate that BMP2,4 and FN play the important roles during bud stage and bell stage,and there may be synergy between BMP-2,4 and FN in regulating differentiation and maturity of odontoblasts.