| 大鼠上下颌突未人化外胚间充质细胞的体外培养 |
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| 引用本文： | 陆怀秀,赵宇,肖明振,牛忠英,郭红延.大鼠上下颌突未人化外胚间充质细胞的体外培养[J].牙体牙髓牙周病学杂志,2001,11(3):143-145. |
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| 作者姓名： | 陆怀秀 赵宇 肖明振 牛忠英 郭红延 |
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| 作者单位： | 第四军医大学口腔医学院, |
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| 摘 要： | 目的：建立体外培养大鼠上下颌突未分化外胚间充质细胞的方法。方法：于饲养细胞上用组织块培养法作原代培养细胞；免疫组织化学法鉴定细胞来源。结果：培养的细胞呈单层贴壁生长，细胞起源鉴定为外胚间充质细胞。结论：建立了一种简便培养大鼠未分化外胚间充质细胞的方法，为下一步的研究工作奠定基础。
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| 关 键 词： | 细胞培养 外胚间充质细胞 免疫组织化学法 饲养层 |
| 文章编号： | 1005-2593(2001)03-0143-03 |
| 修稿时间： | 2000年11月29 |
Culture of mandibular and maxilla ectomesenchymal cells from the embryonic rat |
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| LU Huai-xiu,ZHAO Yu,XIAO Ming-zhen,et al.Culture of mandibular and maxilla ectomesenchymal cells from the embryonic rat[J].Chinese Journal of Conservative Dentistry,2001,11(3):143-145. |
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| Authors: | LU Huai-xiu ZHAO Yu XIAO Ming-zhen |
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| Abstract: | AIM:To cultivate mandibular and maxillary ectomesenchymal cells from the embryonic rat. METHODS: Tissue explant technique was used.Cells were identified by immunohistochemical method. RESULTS: The cultured cells displayed monolayer growth.That were ectomesenchymal oringin,which was proved by immunohistochemical stain. CONCLUSION: It is a simple method to cultivate ectomesenchymal cells from embryonic rat. |
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| Keywords: | cell culture ectomesenchymal cells immunohistochemical method feeder layer |
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