| 一株产果胶酶荷叶内生真菌的分离鉴定 |
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| 引用本文： | 赵晓璐,周 宁,唐咸来,谢庆武.一株产果胶酶荷叶内生真菌的分离鉴定[J].中国酿造,2016,35(8):91. |
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| 作者姓名： | 赵晓璐 周 宁 唐咸来 谢庆武 |
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| 作者单位： | 1.广西大学生命科学与技术学院，广西南宁530004；2.亚热带农业生物资源保护与利用国家重点实验室，广西南宁530004；
3.广西壮族自治区科学技术厅，广西南宁530022 |
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| 基金项目： | 广西壮族自治区研究生教育创新计划项目（P3130098105） |
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| 摘 要： | 以果胶为唯一碳源，通过刚果红染色法从荷叶中筛出1株产果胶酶的内生真菌HY2。根据形态学特征与ITS序列分析，将菌株HY2鉴定为炭疽菌属(Colletotrichum sp.)。该菌在28 ℃、150 r/min条件下发酵培养10 d，经3,5-二硝基水杨酸法(DNS)法测定发酵液中果胶酶酶活为62.9 U/mL。
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| 关 键 词： | 荷叶 内生真菌 炭疽菌属 果胶酶 |
Isolation and identification of pectinase-producing endophytic fungus from lotus leaves |
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| ZHAO Xiaolu,ZHOU Ning,TANG Xianlai,XIE Qingwu.Isolation and identification of pectinase-producing endophytic fungus from lotus leaves[J].China Brewing,2016,35(8):91. |
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| Authors: | ZHAO Xiaolu ZHOU Ning TANG Xianlai XIE Qingwu |
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| Affiliation: | 1.College of Life Science and Technology, Guangxi University, Nanning 530004, China; 2.State Key Laboratory of Conservation and Utilization of Agricultural Bioresources in the Subtropics, Nanning 530004, China; 3.The Science and Technology Department of Guangxi, Nanning 530022, China |
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| Abstract: | With pectin as the sole carbon source, a pectinase-producing endophytic fungus HY2 was screened from lotus leaves by Congo red staining method. According to morphological characteristics and ITS sequence analysis, the strain HY2 was identified as Colletotrichum sp. Under the conditions of temperature 28 ℃, rotate speed 150 r/min and fermentation time 10 d, the pectinase activity in the fermentation liquid was 62.9 U/ml by 3,5-dinitrosalicylic acid method. |
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| Keywords: | lotus leaves endophytic fungus Colletotrichum sp pectinase |
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