| D-101大孔吸附树脂纯化玉米须总黄酮工艺研究 |
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| 引用本文： | 包京姗,韩凤波,毕 博,杨世海.D-101大孔吸附树脂纯化玉米须总黄酮工艺研究[J].中国酿造,2016,35(5):171. |
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| 作者姓名： | 包京姗 韩凤波 毕 博 杨世海 |
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| 作者单位： | 1.吉林农业大学中药材学院，吉林长春130118；2.吉林农业科技学院，吉林吉林132101 |
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| 基金项目： | 吉林农业科技学院中药学省级重点学科培育资助项目（吉农院合字2014第z016号） |
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| 摘 要： | 通过超声波提取玉米须总黄酮，采用D-101大孔树脂进行分离纯化，得到最佳工艺参数：5 g大孔吸附树脂，玉米须总黄酮上样液质量浓度7 mg/mL，体积1 mL，以HCl调节pH=3，体积分数60%乙醇洗脱，洗脱体积5 BV，流速1.0 mL/min。在此条件下，玉米须总黄酮的纯度由41.35%提高到69.20%。
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| 关 键 词： | D-101大孔吸附树脂 玉米须 总黄酮 纯化 |
Purification process of total flavonoid from Stigma maydis by D-101 macroporous adsorption resins |
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| BAO Jingshan,HAN Fengbo,BI Bo,YANG Shihai.Purification process of total flavonoid from Stigma maydis by D-101 macroporous adsorption resins[J].China Brewing,2016,35(5):171. |
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| Authors: | BAO Jingshan HAN Fengbo BI Bo YANG Shihai |
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| Affiliation: | 1.College of Traditional Medicine, Jilin Agricultural University, Changchun 130118, China;
2.Jilin Agricultural Science and Technology University, Jilin 132101, China |
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| Abstract: | Total flavonoids were extracted from Stigma maydis by ultrasonic extraction, and then they were separated and purified by D-101 macroporous adsorption resin. The optimal extraction condition was that D-101 macroporous adsorption resin 5 g, samples concentration 7.0 mg/ml, volume 1 ml, then adjust to pH 3 with HCl, elute with concentration 60% ethanol, with elute colume 5 BV and velocity 1.0 ml/min. The purification degree of total flavonoids in S. maydis increased from 41.35% to 69.20%. |
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| Keywords: | D-101 macroporous adsorption resin Stigma maydis total flavonoids purification |
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