烟草野火病病原菌对农用链霉素的抗药性测定

为合理防治烟草野火病，减少抗药性产生，减少农药残留，从云南省8个地州分离烟草野火病菌菌株43个，并就分离菌株对农用链霉素的抗药性进行了测定，结果表明，供试43个菌株中高抗菌株8个，中抗菌株比例较高17个，低抗菌株18个。不同来源的烟草野火病原菌株的抗药性存在差异，文山、昆明的菌株抗药性最高，红河菌株抗药性最低，抗性倍数最高达2000多倍，其余地州抗药性居中。不同地州分离菌株高、中、低抗菌株的比例存在明显差别。分离自文山邱北县双龙营镇的25#菌株抗药性最强，抗性倍数达45907.75，来自文山砚山县阿猛镇租白村的34#菌株抗药性最低。43株烟草野火病菌菌株对农用链霉素的EC50值平均为4.65，范围在0.004~183.631。高、中、低抗菌株的平均抗性倍数分别为6124.69、49.72、6.40。

Determination of Pseudomonas syringae pv. tabaci Resistance to Agricultural Streptomycin

To control tobacco wild fire disease rationally, reduce resistance and reduce pesticide pollution, 43 strains of Pseudomonas syringae pv. tabaci were isolated from 8 region of Yunnan province and all isolates were determined their resistance to agricultural streptomycin. The results indicated that of all tested isolates, there were 8 high resistance, 17 middle resistance and 18 low resistance isolates respectively. The isolates fungicide resistance from different region was different obviously, the highest resistant isolate come from Wenshan and Kunming region, Honghe isolates had the lowest resistance among all tested isolates. The evident disparity of high-resistance, mid-resistance and low-resistance isolates ratio was exist at different region. 25# from Wenshan Qiubei was the highest agricultural streptomycin resistant isolate, its resistance multiple arrived to 45908 compared to the lowest resistance isolate 34#, which from Yanshan of Wenshan. The EC50 of 43 Pseudomonas syringae pv. tabaci isolates against agricultural streptomycin was 4.65 in average, was in the range of 0.004~183.631. The average resistance multiple of high-resistance, mid-resistance and low-resistance isolates was 6124.69, 49.72, 6.40 respectively.