| 实生成年苹果树外植体的快速微型繁殖 |
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| 引用本文： | 黄仕周,刘艾琴.实生成年苹果树外植体的快速微型繁殖[J].云南植物研究,1986(2). |
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| 作者姓名： | 黄仕周 刘艾琴 |
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| 作者单位： | 中国科学院昆明植物研究所
(黄仕周)，中国科学院昆明植物研究所(刘艾琴) |
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| 摘 要： | 试验结果表明:以实生成苹果树上的芽作为外植体,培养在添加1—2mg/l 6-BA的SH或MS培养基上,在继代培养时芽的萌发率达92％以上,50天内芽数增殖5—6.8倍;同期内,液体旋转培养比琼脂静置培养芽条长度要增加3倍左右;芽条在0.2—0.4mg/lIBA中生根率80％以上;试管苗的移栽成活率85％以上。
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| 关 键 词： | 快速微型繁殖 实生成年苹果树 |
RAPID MICRO-PROPAGATION OF EXPLANTS FROM ADULT SEEDLING APPLE TREES IN VITRO |
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| Huang Shizhou,Liu Aiqin.RAPID MICRO-PROPAGATION OF EXPLANTS FROM ADULT SEEDLING APPLE TREES IN VITRO[J].Acta Botanica Yunnanica,1986(2). |
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| Authors: | Huang Shizhou Liu Aiqin |
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| Abstract: | The experimental result showed. In the medium of Murashige and Shoog (MS) or Schenk and Hidebrandt (SH) added with 6 -benzylamino-purine ( 6 -BA) at 1-2 mg/1, the sprouting rate of buds in subculture of the buds from the adult seedling apple trees were over 92%. Buds multiplied 5-6.8 time in fifty days. The length of shoots in a rotating liquid culture was about three times more than that in the culture on agar-medium. In the medium added with indole-3-butyric arid (IBA) at 0.2-0.4 mg/1, the rooting rate of shoots were over 80%. The survival rate of transplanting tuble-plantlets were more than 85%. |
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| Keywords: | Rapid micro-propagation Adult seedling apple trees |
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