HPLC测定藏药六味能消散中蒽醌类成分含量

目的建立测定藏药六味能消散中蒽醌类成分芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量测定方法。方法采用高效液相色谱法。色谱柱为Eclipse XDB C18柱(150 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸溶液(88∶12),检测波长为254 nm,柱温为30℃,流速为1.0 mL/min。结果芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚分别在0.076~0.380μg(r=0.999 6)、0.068~0.340μg(r=0.999 8)、0.076~0.380μg(r=0.999 9)、0.070~0.349μg(r=0.999 9)、0.071~0.355μg(r=0.999 7)范围内与各自峰面积积分值呈良好线性关系,平均加样回收率分别为97.88%(RSD=0.72%)、97.52%(RSD=0.96%)、98.79%(RSD=0.95%)、97.77%(RSD=1.18%)、96.34%(RSD=2.00%)。结论本方法简便、准确、重复性好,可用于藏药六味能消散的质量控制。

Content Determination of Anthraquinone in Tibet Traditional Medicine Liuwei Nengxiaosan by HPLC

Objective To set up a method for determining the content ot antnraqulnone in LiUWel Nengxiaosan. Methods The chromatogram column was Eclipse XDB C18 （150 mm X 4.6 mm, 5μm）, the mobile phase composition was methanol-0.1% orthphosphoric acid （88 ： 12）, the detection wavelength was at 254 nm, the column temperature was 30 ℃, the flow rate was 1.0 mL/min. Results The linear range of aloe emodin, rhein, emodin, chrysophanol and physcion was 0.076-0.380 μg （r=0.999 6）, 0.068- 0.340 μg （r=0.999 8）, 0.076-0.380μg （r=0.999 9）, 0.070-0.349 μg （r=0.999 9）, 0.071-0.355 μg （r=0.999 7）, respectively. The average recovery rate respectively was 97.88% （RSD=0.72%）, 97.52% （RSD=0.96%）, 98.79% （RSD=0.95%）, 97.77% （RSD= 1.18%）, 96.34% （RSD =2.00%）. Conclusion The method is highly effective, quick and sensitive, suitable for quality control of Tibet traditional medicine Liuwei Nengxiaosan.