| 微生物浸出Pb-Zn-Sn黄铜矿及其生物群落的演替分析 |
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| 引用本文： | 王军,赵红波,庄田,覃文庆,朱珊,邱冠周.微生物浸出Pb-Zn-Sn黄铜矿及其生物群落的演替分析[J].中国有色金属学会会刊,2013,23(12):3758-3762. |
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| 作者姓名： | 王军 赵红波 庄田 覃文庆 朱珊 邱冠周 |
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| 作者单位： | 中南大学资源加工与生物工程学院,长沙410083中南大学生物冶金教育部重点实验室,长沙410083 |
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| 基金项目： | Project （51374248） supported by the National Natural Science Foundation of China，Project （NCET-13-0595） supported by the Program for New Century Excellent Talents in University, China，Project （2012AA061501） supported by the High-tech Research and Development Program of China，Project （2010CB630905） supported by the National Basic Research Program of China，Project （20120162120010） supported by the Research Fund for the Doctoral Program of Higher Education of China，Project （CSUZC2012020） supported by the Open-End Fund for the Valuable in Central South University, China |
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| 摘 要： | 研究了Pb-Zn-Sn黄铜矿精矿在混合中度嗜热微生物槽浸过程中的细菌群落结构变化，并监测浸出体系中金属离子浓度、溶液电位、溶液pH值变化，通过聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）技术分析微生物群落的结构变化。结果表明，最终铜浸出率高达85.6%，在浸出前期，Acidithiobacillus caldus为优势群落，从第18天开始到浸出结束，Sulfobacillus thermosulfidooxidans为优势群落，但Leptospirillum ferriphilum丰度变化较小。试验结果表明，适当较高的溶液电位和合适的铁离子浓度对黄铜矿精矿的生物浸出作用很关键。
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| 关 键 词： | 黄铜矿 微生物槽浸 微生物群落 聚合酶链式反应-限制性片段长度多态性技术PCR-RFLP |
| 收稿时间： | 20 March 2013 |
Bioleaching of Pb-Zn-Sn chalcopyrite concentrate in tank bioreactor and microbial community succession analysis |
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| Jun WANG,Hong-bo ZHAO,Tian ZHUANG,Wen-qing QIN,Shan ZHU,Guan-zhou QIU.Bioleaching of Pb-Zn-Sn chalcopyrite concentrate in tank bioreactor and microbial community succession analysis[J].Transactions of Nonferrous Metals Society of China,2013,23(12):3758-3762. |
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| Authors: | Jun WANG Hong-bo ZHAO Tian ZHUANG Wen-qing QIN Shan ZHU Guan-zhou QIU |
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| Affiliation: | 1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; 2. Key Laboratory of Biohydrometallurgy of Ministry of Education, Central South University, Changsha 410083, China) |
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| Abstract: | The variation of microbial community structure was investigated for the tank bioleaching process of Pb-Zn-Sn chalcopyrite concentrate in the presence of mixed moderately thermophilic bacteria. The parameters, such as pH value, solution potential and concentrations of metal ions, were determined by the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to analyze the succession of microbial community. The results showed that a final copper extraction rate of 85.6% could be obtained after tank bioleaching for 30 d. The Acidithiobacillus caldus was the dominant population with abundance of about 73.80%in the initial stage, then Sulfobacillus thermosulfidooxidans dominated from the 18th day to the end of bioleaching, while the abundance of Leptospirillum ferriphilum changed slightly. A higher solution potential within a certain range and appropriate concentration of ferric ions were essential for this tank bioleaching of chalcopyrite. |
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| Keywords: | chalcopyrite tank bioleaching microbial community PCR-RFLP technique |
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