| 金鱼胚胎蛋白质双向凝胶电泳方法建立及优化 |
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| 引用本文： | 喻小燕,陈妍,曹晶,谢锦云.金鱼胚胎蛋白质双向凝胶电泳方法建立及优化[J].金陵科技学院学报,2009,25(2):91-96. |
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| 作者姓名： | 喻小燕 陈妍 曹晶 谢锦云 |
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| 作者单位： | 1. 湖南信息职业技术学院,湖南,长沙,410200;湖南师范大学生命科学学院,湖南,长沙,410081
2. 湖南师范大学生命科学学院,湖南,长沙,410081 |
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| 摘 要： | 以金鱼胚胎为材料,以不同样品制备方式、裂解液成分、固相pH梯度胶条的选择、凝胶浓度为侧重点,建立并优化金鱼胚胎总蛋白质组最佳双向凝胶电泳技术条件.研究表明,采用匀浆器匀浆含硫脲和NP-40裂解液提取蛋白,应用pH值为3～10的非线性IPG胶条和浓度14%的SDS-PAGE凝胶进行双向电泳,经优化电泳电流、时间等参数后,获得了重复性和分辫率理想的金鱼胚胎总蛋白质双向电泳图谱,为金鱼胚胎蛋白质组分析莫定了基础,为揭示金鱼单倍体发育异常的机制提供了技术保障.
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| 关 键 词： | 金鱼胚胎 蛋白质组 双向凝胶电泳 |
Establishment and Optimization of Two-dimensional Gel Electrophoresis Condition for Goldfish Embryos Proteome |
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| YU Xiao-yan,CHEN Yan,CAO Jing,XIE Jin-yun.Establishment and Optimization of Two-dimensional Gel Electrophoresis Condition for Goldfish Embryos Proteome[J].Journal of Jinling Institute of Technology,2009,25(2):91-96. |
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| Authors: | YU Xiao-yan CHEN Yan CAO Jing XIE Jin-yun |
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| Affiliation: | 1.Hunan College of Information;Changsha 410200;China;2.Hunan Normal University;Changsha 410081;China |
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| Abstract: | To develop and optimize a two-dimensional(2-DE) gel electrophoresis condition for goldfish embryos proteome,total protein of goldfish embryos is extracted,and the optimal conditions for sample preparation and 2-DE are screened by comparing the following parameters: embryos broken methods,contents of lysis buffer,the concentration of gel,and linear or non-linear immobilized pH gradient gels.The results demonstrate that a satisfactory 2-DE map with good repetition and resolution is obtained and suitable condi... |
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| Keywords: | goldfish embryos proteome two-dimensional gel electrophoresis |
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