复方仙贞汤对去卵巢大鼠模型骨密度和骨形态的影响

目的 研究复方仙贞汤对去卵巢大鼠的骨密度和骨形态的影响。方法 3月龄的雌性SD大鼠，普通饲料喂养1周后，随机分为假手术组（Sham）、模型组（OVX）、尼尔雌醇阳性药物对照组、复方仙贞汤小剂量组、复方仙贞汤中剂量组、复方仙贞汤大剂量组。除假手术组，其余都经背部切开后切除双侧卵巢，制造去卵巢模型（OVX），假手术组则背部切开后切除部分脂肪组织再分层缝合。造模完成后，假手术组及模型组用生理盐水灌胃，阳性药物组用尼尔雌醇混悬液灌胃，复方仙贞汤小、中、大剂量组分别给以6．5g／kg、13g／kg、26g／kg大鼠体重（按生药计算）的复方仙贞汤药液，每日固定上午九点灌胃1次。全部动物共持续喂养3个月后处死，测定股骨的骨密度和胫骨骨体积、胫骨类骨表面，类骨质宽、骨吸收表面。结果 复方仙贞汤组和尼尔雌醇组均较模型组的骨密度显著性增高（P〈0．01），而且复方仙贞汤中剂量组与尼尔雌醇阳性对照组接近。与模型组比较，中药组和尼尔雌醇组的骨体积显著上升（P〈0．01），而类骨表面、类骨质宽和骨吸收表面却显著下降（分别P〈0．01）。结论 复方仙贞汤能提高去卵巢大鼠的骨密度和骨体积，减少类骨质宽、类骨质表面、骨吸收表面，提示复方仙贞汤能起到和尼尔雌醇相似的作用，即降低绝经后骨质疏松的高转换率，从而减慢骨质的丢失。

Effect of Compound Xinnzhen Decoction on bone mineral density and bone morphology in ovariectomized osteoporosis rats

Objective To study the effect of Compound Xianzhen Decoction on bone mineral density and bones morphology in ovariectomized osteoporosis rats. Methods The female Sprague-Dawley (SD) rats, three months old, were randomly selected as Sham-operated group, ovariectomized group(OVX group), Nilestriol group, Compound Xianzhen Decoction at low, medium and large dose group, respectively. All rats were ovariectomized except for those in the Sham-operated group. After operation, the rats in the Shamed group and OVX group were fed with sodium chloride, the Nilestriol group with Nilestriol suspension, the Xianzhen Decoction at low, medium and large dose group with Xianzhen Decoction at 5.98 g/kg, 11.96 g/kg and 23.92 g/kg, respectively. All animals were killed after 3 months and the femoral bone mineral density and humeral volume, osteoid width,osteoid surface and the surface of bone absorption were tested. Results The bone mineral density of the Compound Xianzhen Decoction group and Nilestriol group increased significantly(P<0.01). However, the result of the medium dosage of Compound Xianzhen Decoction group was close to that of Nilestriol group. Compared with the model group,the bone volume in Chinese medicine and Nilestriol group increased significantly(P<0.01), but the bone volume, osteoid width of osteoid surface and the surface of bone absorption decreased significantly (P<0.01). Conclusions Compound Xianzhen Decoction can increase the bone mineral density and bone volume in ovariectomized rats. At the same time, it reduces the osteoid width, osteoid surface and the surface of bone absorption. Compound Xianzhen Decoction has the similar founction as Nilestriol, that is, reducing the higher conversion rate of osteoblast and osteoclast in postmenopausal osteoporosis and slowing down the bone loss.