不同取血方法和动静脉血对Wistar大鼠生化指标的影响

目的观察断头取血与腹主动脉取血及腹主动脉血与腹主静脉血对Wistar大鼠生化指标的影响,为毒理学试验中的取血方法和取血部位的选择提供依据和参考。方法选择Wistar大鼠80只(雌/雄各40只),按体质量随机分为断头取血组和腹主动/静脉取血组,用全自动生化分析仪测定血清TP、ALB、A/G、ALT、AST、BUN、CRE、CHO、TG、GLU等指标。结果两种取血方法在两种性别大鼠中,上述测定指标均存在不同程度的差异:两种性别大鼠断头取血组A/G、CRE、TP、ALB、ALT、AST、CHO、TG都高于腹主动脉组,差异有统计学意义(P0.05);GLU为断头组偏低,雌性大鼠断头取血组BUN高于腹主动脉组,两项指标均差异有统计学意义(P0.05)。动静脉取血产生的影响主要体现在TG、GLU等生化指标中,两种性别大鼠腹主动脉组TG含量较低,而GLU含量只有雄性大鼠腹主动脉组高于腹主静脉组,两项指标均差异有统计学意义(P0.05)。结论不同的取血方法及动静脉血对Wistar大鼠的生化指标会产生明显的影响。

Influence of different blood sampling methods and the difference of arterial blood and venous blood samples on biochemical indicators in Wistar rat

To investigate the difference of biochemical indicators between decapitated blood, abdominal aortic blood and abdominal venous blood on in Wistar rat, and to provide the basis and reference of blood sampling method for toxicology tests.Methods 40 male and 40 female Wistar rat were divided into decapitated blood group and abdominal aortic/venous blood group. Serum parameters of TP, ALB, A/G, ALT, AST, BUN, CRE, CHO, TG, GLU were detected by automatic biochemical analyzer.Results All parameters were differences between these two blood sampling methods in two kinds of gender. The broken blood group in TP, ALB, A/G(female P<0.05), ALT, AST, CRE(female P<0.05), CHO, TG exceed the abdominal aortic blood group obviously on two sex(P<0.05), lower glucose in the broken blood group(P<0.05), BUN in the broken blood group exceed abdominal aortic blood group on female (P<0.05). Influences of aortic blood and venous blood embedded in serum parameters of TG, GLU, TG in the abdominal aortic blood group was lower on two sex(P<0.05), GLU in the abdominal aortic blood group exceed the abdominal venous blood group obviously on male(P<0.05), no difference in female.Conclusion There are significant influences on biochemistry parameters between different blood sampling methods and arterial blood and venous blood in Wistar rat.