中重度阻塞性睡眠呼吸暂停低通气综合征患者外周血内皮祖细胞数量和功能的变化

目的〖KG*2〗探讨中重度阻塞性睡眠呼吸暂停低通气综合征（obstructive sleep apnea hypopnea syndrome,OSAHS）患者外周血中内皮祖细胞数量和功能的变化。 〖HTH〗方法〖KG*2〗采用前瞻性研究方法，收集中重度OSAHS成人患者30例为OSAHS组，同时选择健康人30例为对照组，无菌空腹采集10 mL 新鲜静脉全血，采用淋巴分离液分离及密度梯度离心法提取单核细胞，以内皮祖细胞（endothelial progenitor cells,EPCs）培养基培养，使用荧光显微镜鉴定内皮祖细胞；评估2组人群内皮祖细胞数目、迁移能力、增殖能力、黏附能力。 〖HTH〗结果〖KG*2〗OSAHS组EPCs数量、吸光度值、迁移细胞数及黏附率显著低于对照组，差异有统计学意义(P＜0.05)。 〖HTH〗结论〖KG*2〗和正常人群比较，中重度OSAHS患者外周血内皮祖细胞数目减少，增殖能力、迁移能力和黏附能力下降。

Changes of endothelial progenitor cells in peripheral blood of patients with obstructive sleep apnea hypopnea syndrome

ObjectiveTo study the changes of endothelial progenitor cells in peripheral blood of patients with obstructive sleep apnea hypopnea syndrome（OSAHS）. MethodsA prospectivestudy of 30 obstructive sleep apnea hypopnea syndrome patients were selected in the department of otolaryngology, the first hospital of Hebei Medical University. Thirty healthy subjects were selected as control group. Monocytes were obtained from 10 mL of venous blood by density gradientcentrifugation, Endothelial progenitor cells(EPCs）were cultured and identified byfluorescence microscopy. The number, migration, proliferation and adhesion of EPCs in two groups were evaluated. ResultsThe number of proliferating cells, migratingcellsand adheringratein the normal control group were significantlyhigher than those in the OSAHS group(P＜0.05). ConclusionThe number of endothelial progenitorcells in OSAHS patients with hard plaque is small, and their proliferation, migration andadhesion ability are impaired.