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沙门菌检测方法的循证确定
引用本文:沈圣,江华洲,邱晋,许欣,裴晓方.沙门菌检测方法的循证确定[J].预防医学情报杂志,2006,22(1):106-109.
作者姓名:沈圣  江华洲  邱晋  许欣  裴晓方
作者单位:1. 四川大学华西医学中心公共卫生学院医检教研室,四川,成都,610041
2. 四川大学口腔生物医学工程教育部重点实验室
摘    要:目的将循证医学的研究方法应用于卫生检验,寻找沙门菌快速检测的最佳方案。方法通过检索多个数据库,全面查找沙门菌检测方法的文献,并对找到的文献进行评估和筛选,找到符合要求的文献确定检测方法。结果从5256篇检索到的文献中,共找到相关Ⅱ级文献4篇,Ⅲ级综述文献1篇用于确定沙门菌快速检测的最佳方案。结论在沙门菌的快速检测中,宜采用以139-141为引物的PCR检测法。

关 键 词:沙门菌  循证医学  聚合酶链反应
文章编号:1006-4028(2006)01-106-04
收稿时间:2005-12-23
修稿时间:2005年12月23

Confirmation of Detection Method of Salmonella by Applying Study Methodology of Evidence - based Medicine (EBM)
Shen Sheng, Jiang Hua-zhou, Qiu Jin.Confirmation of Detection Method of Salmonella by Applying Study Methodology of Evidence - based Medicine (EBM)[J].Journal of Preventive Medicine Information,2006,22(1):106-109.
Authors:Shen Sheng  Jiang Hua-zhou  Qiu Jin
Affiliation:Department of Laboratory Medicine, Public Health School of Sichuan University, Chengdu 610041, China
Abstract:Objective To find out the optimal technique for rapid detection of Salmonella by applying study m ethodology of evidence-based medicine (EBM). Methods Corresponding references in several databases were searched and evaluated across-the-board in order to find out the best rapid detection method for Salmonella. Results Among 5 256 articles, 4 second-class and 1 third-class corresponding references were found. Conclusions PCR method in which 139-141 as primer is suitable for rapid detection of Salmonella.
Keywords:Salmonella  EBM  Polymerase Chain Reaction
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