| Simultaneous determination of adenine, uridine and adenosine in cordyceps sinensis and its substitutes by LC／ESI-MS |
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| 引用本文： | 黄兰芳,吴名剑,孙贤军,郭方遒,梁逸曾,李晓如.Simultaneous determination of adenine, uridine and adenosine in cordyceps sinensis and its substitutes by LC／ESI-MS[J].中南工业大学学报(英文版),2004,11(3):295-299. |
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| 作者姓名： | 黄兰芳 吴名剑 孙贤军 郭方遒 梁逸曾 李晓如 |
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| 作者单位： | School of Chemistry and Chemical Engineering，Central South University，Changsha 410083，China，Technical Center of Changde Cigarette Factory，Changde 415000，China，Technical Center of Changde Cigarette Factory，Changde 415000，China，School of Chemistry and Chemical Engineering，Central South University，Changsha 410083，China，School of Chemistry and Chemical Engineering，Central South University，Changsha 410083，China，School of Chemistry and Chemical Engineering，Central South University，Changsha 410083，China |
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| 摘 要： | A simple, sensitive and reproducible high performance liquid chromatography-mass spectrometry coupled with electrospray ionization method for simultaneous separation and determination of adenine, adenosine and uridine was developed. The analytical column is a 2.0 mm× 150 mm Shimadzu VP-ODS column and volume fraction of the mobile phase is 86.5 %water, 12.0%methanol and 1.5%formic acid. 2-chloroadenosine was used as internal standard. Selective ion monitoring mode and selective ion monitoring ions at ratio of mass to electric charge of 136 for adenine, 268 for adenosine and 267 for uridine were chosen for quantitative analysis of the three active components. The results show that the regression equations and linear range are Y=0. 062X 0. 005 and 2.0 - 140.0μg · mL 1for adenine, Y=0. 049X 0. 004 and 4. 0 - 115.0 μg · mL-1 for uridine, Y=0. 154X 0. 014 and 1.0 - 125.0 μg · mL 1 for adenosine. The limits of detection are 0.6 μg · mL 1 for adenine, 1.0μg · mL-1 for uri dine and 0.2 μg · mL 1 for adenosine.The recoveries of the three constituents are from 96.6% to 103.2%.
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| 关 键 词： | 腺嘌呤 质谱分析 静电 电离 虫草素 核苷 |
| 收稿时间： | 4 July 2003 |
| 修稿时间： | 25 September 2003 |
Simultaneous determination of adenine, uridine and adenosine in cordyceps sinensis and its substitutes by LC/ESI-MS |
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| Huang Lan-fang , Wu Ming-jian , Sun Xian-jun , Guo Fang-qiu , Liang Yi-zeng and Li Xiao-ru.Simultaneous determination of adenine, uridine and adenosine in cordyceps sinensis and its substitutes by LC/ESI-MS[J].Journal of Central South University of Technology,2004,11(3):295-299. |
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| Authors: | Huang Lan-fang Wu Ming-jian Sun Xian-jun Guo Fang-qiu Liang Yi-zeng and Li Xiao-ru |
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| Affiliation: | (1) School of Chemistry and Chemical Engineering, Central South University, 410083 Changsha, China;(2) Technical Center of Changde Cigarette Factory, 415000 Changde, China |
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| Abstract: | A simple, sensitive and reproducible high performance liquid chromatography-mass spectrometry coupled with electrospray ionization
method for simultaneous separation and determination of adenine, adenosine and uridine was developed. The analytical column
is a 2.0 mm × 150 mm Shimadzu VP-ODS column and volume fraction of the mobile phase is 86.5% water, 12.0% methanol and 1.5%
formic acid. 2-chloroadenosine was used as internal standard. Selective ion monitoring mode and selective ion monitoring ions
at ratio of mass to electric charge of 136 for adenine, 268 for adenosine and 267 for uridine were chosen for quantitative
analysis of the three active components. The results show that the regression equations and linear range are Y=0.062X+0.005 and 2.0 – 140.0 μg·mL−1 for adenine, Y=0.049X+0.004 and 4.0–115.0 μg · mL−1 for uridine, Y=0.154X+0.014 and 1.0–125.0 μm · mL−1 for adenosine. The limits of detection are 0.6 μg·mL−1 for adenine, 1.0 μg·mL−1 for uridine and 0.2 μg·mL−1 for adenosine. The recoveries of the three constituents are from 96.6% to 103.2%.
Foundation item: Project (20175036 and 20235020) supported by the National Natural Science Foundation of China |
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| Keywords: | high performance liquid chromatography electrospray ionization interface mass spectrometry cordyceps sinensis nucleoside |
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