紫外分光光度法测定吩嗪-1-羧酸的含量

目的]建立紫外分光光度法测定生物农药吩嗪-1-羧酸(phenazine-1-carboxylic acid,PCA)含量的方法。方法]PCA经有机溶剂溶解,微孔滤膜过滤后,在波长249 nm处用紫外分光光度法定量。结果]乙腈是4种常用有机溶剂(乙腈、乙酸乙酯、甲醇、乙醇)中溶解PCA的最佳溶剂,线性质量浓度范围为0.25~3.0 mg/L(r2为0.999 9),相对标准偏差(RSD)最大为4.5%,检出限为0.01 mg/L,加标回收率为95.0%~101.9%。结论]与高效液相法比较,该方法准确度、精密度良好,操作简单,易于推广使用。

Ultraviolet Spectrophotometric Method for the Determination of Phenazine-1-carboxylic Acid

Aims] A novel ultraviolet spectrophotometric method was established for the determination of phenazine-1carboxylic acid(PcA).Methods] In the process,PCA was dissolved in organic solvent,filtrated through millipore filter and then detected by UV spectrophotometer at 249 nm.Results] Acetonitrile was the optimal choice in the four organic solvents of acetonitrile,ethyl acetate,methanol and ethanol.The linearity was within 0.25-3.0 mg/l with high relativity of 0.9999.The recovery rates were 95.0-101.9% and rsD was lower than 4.5%.The lowest detection limit was 0.01 mg/l.Conclusions] The method was characterized by convenient operation,high accuracy and precision,and was suitable for wide usage.