马兰叶总黄酮的提取及其含量的测定

优化了马兰叶总黄酮的超声提取工艺.结果表明,最佳的提取工艺是：乙醇体积分数为80％,提取温度50℃,料液比1/50（g/mL）,提取时间50 min,总黄酮的提取率为7.01％.以氯化铝为显色剂、芦丁为标准品,用紫外分光光度法测定黄酮含量.检测波长为418 nm,芦丁浓度在0.004～0.100 mg/mL,有良好的线性关系（R2=0.998 6）,平均加样回收率为97.4％（RSD=0.96％,n=5）,该方法简单、重复性好、结果可靠,适于马兰叶总黄酮的含量测定.

Extraction and determination of total flavonoids in leaves of Kalimeris indica （Linn.） Sch.

Ultrasonic assisted extraction of total flavonoids from Kalimeris indica （ Linn. ） Sch. leaves was optimized. The optimum conditions of extraction process were ethanol 80% , extraction temperature 50 ℃, the ratio of solid to solvent 1 ： 50 （ g ： mL）, extraction time 50 rain, and the best extraction yield was 7.01%. Content of total flavonoids was determined by spectrophotometry. Rutin was used as standard sample and aluminum chloride-methanol solution as the chromagenic reagent. The results showed detection wavelength was 418 nm, a good linear relationship was obtained in the range of 0. 004 - 0. 100 mg/mL （ R2 = 0. 998 6）, and the average recovery was 97.4% （ RSD = 0.96%, n = 5 ）. The method is simple, easy and reproducible, results are reliable, suitable for the amount determination of total flavonoids in leaves of Kalimerls indica （Linn.） Sch.