抑制Ppif基因的表达对大鼠肾缺血再灌注损伤的保护作用

目的 观察抑制Ppif基因的表达对缺血再灌注损伤肾脏的保护作用,并探讨其作用机制.方法 建立大鼠肾缺血再灌注模型,将实验动物随机分为3组:空白对照组、阴性对照组、治疗组(各20只).治疗组大鼠给Ppif基因靶向RNA干扰(RNAi)慢病毒载体(4μg)0.3 ml,阴性对照组再灌注时给予0.3 ml含体积分数0.01二甲基亚砜(DMSO)的生理盐水,空白对照组不夹闭肾蒂,余处理同阴性对照组.分别检测各组血肌酐(Cr)含量、尿素氮(BUN)含量、细胞凋亡指数(AI)、苏木素-伊红(HE)染色组织病理学分析.结果 治疗组与阴性对照组比较,血Cr和BUN均明显降低,AI明显下降,差异均有统计学意义(P＜0.05),HE染色组织病理学分析结果显示治疗组较另外2组缺血明显减轻.结论 Ppif基因靶向RNAi慢病毒载体能抑制大鼠Ppif基因的表达,从而抑制线粒体的凋亡,减轻肾脏缺血再灌注损伤.

Abstract：

Objective To study the protective effect of Ppif gene inhibitor on renal ischemiareperfusion injury and the action mechansim. Methods A renal ischemia-reperfusion model was made in 60 rats, and the rats were evenly divided into three groups: control group (CON group), negative control group (NC group), and Ppif gene inhibitor group (treated group). The blood urea nitrogen (BUN) and creatinine (Cr), and renal cell apoptosis index (AI) were measured. Histopathological analysis was performed by using HE staining. Results A comparison between treated group and NC group showed that for treated group, the levels of both Cr and BUN were decreased, and AI decreased in the treated group as compared with the NC group (P ＜ 0. 05 ). Histolopathological analysis revealed that the ischemia in the treated group was significantly alleviated as compared with other two groups. Conclusion Ppif gene-targeted RNA interference ( RNAi ) lentiviral vector could inhibit the expression of Ppif gene in rats, thereby inhibiting the mitochondrial apoptosis and alleviating renal ischemia-reperfusion injury.

The protective effect of Ppif gene inhibitor on renal ischemia-reperfusion injury

Objective To study the protective effect of Ppif gene inhibitor on renal ischemiareperfusion injury and the action mechansim. Methods A renal ischemia-reperfusion model was made in 60 rats, and the rats were evenly divided into three groups: control group (CON group), negative control group (NC group), and Ppif gene inhibitor group (treated group). The blood urea nitrogen (BUN) and creatinine (Cr), and renal cell apoptosis index (AI) were measured. Histopathological analysis was performed by using HE staining. Results A comparison between treated group and NC group showed that for treated group, the levels of both Cr and BUN were decreased, and AI decreased in the treated group as compared with the NC group (P ＜ 0. 05 ). Histolopathological analysis revealed that the ischemia in the treated group was significantly alleviated as compared with other two groups. Conclusion Ppif gene-targeted RNA interference ( RNAi ) lentiviral vector could inhibit the expression of Ppif gene in rats, thereby inhibiting the mitochondrial apoptosis and alleviating renal ischemia-reperfusion injury.