胞质分裂阻断法及染色体畸变实验检测水质诱变性及两种方法的比较

运用人胚肺细胞胞质分裂阻断法（ＣＹ－ＭＮＴ）及染色体畸变实验（ＳＣＡ）检测了某市水源水及自来水的遗传毒性，并比较了两短测实验方法和相应的反映细胞毒性的指标ＮＤＩ（ｎｕｃｌｅａｒｄｉｖｉｓｉｏｎｉｎｄｅｘ）和ＭＩ（ｍｉｔｏｔｉｃｉｎｄｅｘ）之间的关系。结果表明：该市的水源水及自来水中的有机提取物可不同程度地诱导微核率及染色体畸变率的升高，并且各实验剂量组与ＮＤＩ、ＭＩ有很好的负相关关系，源水的相关系数分别为－０．９０４、－０．９８０，自来水的相关系数分别为－０．８８２、－０．９１８，微核率（ＭＮＢＮ，Ｍｉｃｒｏｎｕｃｌｅａｔｅｄｂｉｎｕｃｌｅａｔｅｄ）与染色体畸变率（ＳＣＡ，Ｓｔｒｕ－ｔｕｒａｌｃｈｒｏｍｏｓｏｍａｌａｂｅｒａｔｉｏｎ）及ＮＤＩ与ＭＩ有很好的相关性，源水的相关系数分别为０．８８６、０．９５８，自来水的相关系数分别为０．９９４、０．９６０。

Comparative Study on Water Induced Micronuclei and Chromosomal Aberrations in Human Embryonic Lung Fibroblast Cells

The paper reported the induction of micronuclei (MN) and structural chromosomal aberration (SCA) in human embryonic lung fibroblast cell (KMB) by water supply. The correlation of SCA and MN, NDI and MI was also reported. The organic extracts of both source and drinking water could induce the SCA and MN formation at different doses. NDI and MI decreased with the increase of sample doses ( r =-0.904 and -0.980 in source water and r =- 0.882 and -0.918 in drinking water, respectively). These data suggested a good correlation between chromosome damage as measured by MNBN and aberrant cells and cytotoxicity as measured by NDI and MI. Correlation coefficients of source and drinking water were 0.886 , 0.958 , 0.994 and 0.960 respectively.