基因芯片筛选Sirt1在小鼠急性呼吸窘迫综合征炎症损伤中相互作用的基因

目的筛选小鼠急性呼吸窘迫综合征炎症损伤中与Sirt1相互作用的基因,探讨Sirt1在急性呼吸窘迫综合征炎症反应中的作用机制。方法购买Sirt1过表达(Tg)小鼠和野生型(WT)小鼠,饲养、繁殖、鉴定新生小鼠基因型;Western Blot鉴定小鼠肺组织Sirt1表达差异;建立ARDS小鼠模型,观察ARDS小鼠肺组织HE染色病理变化,并采用ELISA检测两种ARDS小鼠肺组织IL-6表达差异;采用基因芯片筛选Sirt1在小鼠ARDS炎症损伤中相互作用的基因。结果通过聚合酶链反应鉴定出Tg和WT两种不同基因型的小鼠;免疫印迹法检测小鼠肺组织Sirt1的表达差异结果提示Tg小鼠肺组织Sirt1含量显著高于WT小鼠(P=0.001);不同浓度LPS腹腔注射12 h后小鼠肺组织HE染色病理变化提示随着LPS用量增加,两种小鼠肺组织损伤程度明显增加,且WT-ARDS小鼠的肺组织损伤程度比Tg-ARDS小鼠更为严重;当LPS用量达到20 mg/kg时两组小鼠存活率50%;两种小鼠腹腔注射LPS(15 mg/kg)后,肺组织IL-6的表达随时间推移逐渐呈现逐渐增高的趋势,在3,6,12,24 h WT-ARDS组肺组织IL-6表达浓度显著高于Tg-ARDS组(P0.05),尤其在12 h差异最为显著(P=0.007)。基因芯片筛选出在小鼠ARDS炎症损伤中与Sirt1相互作用的基因有Ubd,Ube2d2b,Olfm4,Il1rl1,Hivep3和Lpar1。结论 Sirt1可能通过调控Ubd,Ube2d2b,Olfm4,Il1rl1,Hivep3和Lpar1的表达减轻ARDS小鼠的炎症损伤。

Screening of genes interacting with Sirt1 in inflammatory injury of acute respiratory distress syndrome in mice by gene chip

Objective To Screen of Sirt1 interacting genes in acute respiratory distress syndrome in mice by gene chip,and to provide a reference for exploring the mechanism of action of Sift1 in acute respiratory distress syndrome.Methods Sirt1 overexpression (Tg) mice and wild type (WT) mice were bought for research,and the genotypes of newborn mice were identified after reproduction.The Sirt1 expression differences in lung tissue of Tg mice and WT mice were test by Western Blot.Construction of ARDS mouse model,the expression of IL-6 in lung tissue of two ARDS mice was detected by ELISA,and pathological changes in lung tissue of ARDS mice by HE staining were observed.Screening of Sirt1 interacting genes in acute respiratory distress syndrome in mice by gene chip.Results Two different genotypes of Tg and WT were identified by polymerase chain reaction (PCR).The expression of Sirt1 in lung tissue of mice which detecting by Western blot suggested that the Sirt1 in lung tissue of Tg mice was significantly higher than that of WT mice (P =0.001).Pathological changes of lung tissue of mice after intraperitoneal injection of LPS after LPS via HE staining prompted that with the increase of LPS dosage,the injury degree of lung tissue of two kinds of mice increased obviously,and the degree of lung injury in WT-ARDS mice was more serious than that of Tg-ARDS mice,and when the dosage of LPS reached 20 mg/kg,the survival rate of the two groups was ＜50％.Two kind of mice were injected intraperitoneally with LPS (15 mg/kg),The expression of IL-6 in lung tissue gradually increased with the passage of time,The expression of IL-6 in lung tissue of 3 h,6 h,12 h,24 h inWT-ARDS group was significantly higher than that in Tg-ARDS group(P＜0.05),morever,the difference was the most significant especially in 12 h(P=0.007).Ubd,Ube2d2b,Olfm4,Il1rl1,Hivep3 and Lpar1 were identified as genes that interact with Sirt1 in mouse ARDS inflammatory lesions by gene chip screening.Conclusion Sirt1 may reduce the inflammatory damage in ARDS mice by regulating the expression of Ubd,Ube2d2b,Olfm4,Il1rl1,Hivep3 and Lpar1.