Enhancement of cell growth by intracellularly expressed herpes simplex virus thymidine kinase |
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| Authors: | Sun Ho Park Yeon Su Yu Jongwon Lee |
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| Affiliation: | (1) School of Chemical Engineering and Materials Engineering, Keimyung University, Taegu, Korea;(2) Department of Biochemistry, School of Medicine, Catholic University of Taegu-Hyosung, Taegu, Korea |
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| Abstract: | A recombinant cell line (NIH3T3:pLtkSN) was made by infecting parental cells (NIH3T3) with a recombinant retrovirus (pLtkSN) encoding herpes simplex virus thymidine kinase (HSVtk) gene. The cells expressing HSVtk (NIH3T3:pLtkSN) grew 2.3 times more than the parental cells (NIH3T3) in Dulbecco's Modified Eagles Media containing 10% (v/v) horse serum. The NIH3T3:pLtkSN cells also showed a significant enhancement in the maximal cell concentration and the specific growth rate even at 2.5% serum concentration. The specific O2 uptake rate of NIH3T3 was 2.1 times greater than that of NIH3T3:pLtkSN. Under both O2-limited and O2-unlimited conditions, it appears that HSVtk plays an important role in enhancing the growth characteristics of animal cells. |
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