Isolation of powerful antioxidants from the medicinal halophyte Limoniastrum guyonianum |
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Authors: | Trabelsi Najla Oueslati Samia Falleh Hanen Waffo-Téguo Pierre Papastamoulis Yorgos Mérillon Jean-Michel Abdelly Chedly Ksouri Riadh |
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Affiliation: | Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj Cédria, BP 901, 2050 Hammam-Lif, Tunisia; Laboratoire de Sciences Végétales, Mycologie et Biotechnologie, Groupe d'Etude des Substances Végétales à Activité Biologique (GESVAB) EA 3675 - UFR Pharmacie, Institut des Sciences de la Vigne et du Vin (ISVV), Université Victor Segalen Bordeaux 2, France. |
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Abstract: | The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of Limoniastrum guyonianum Boiss. (Zita) were investigated in this study. The ethyl acetate phase exhibited a significant antioxidant activity as judged by total antioxidant activity, DPPH test and reducing power. Fractionation of this extract by centrifugal partition chromatography (CPC) using quaternary Arizona solvent systems composed of n-heptane/ethyl acetate/methanol/water led to ten fractions. The antioxidant capacities of these fractions were assessed using the same previous tests. Fraction 8 showed the highest antioxidant capacity (1291.1mg GAE/g DR), the power ability to quench DPPH radical (IC(50)=2μg/ml) and to reduce Fe(3+) (EC(50)=65μg/ml). From this fraction, three powerful flavonoids were isolated (1-3): gallocatechin (1), epigallocatechin (2) and epigallocatechin-3-O-gallate (3). These findings suggest that the antioxidative property of L. guynianum is may be related to the presence of these flavonoids, which can be used in various industrial fields. |
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Keywords: | DPPH 2 2-diphenyl-1-picrylhydrazyl IC50 Inhibition Concentration at 50 percent EC50 effective concentration at which the absorbance was 0 5 GAE gallic acid equivalents BHT butylated hydroxytoluene BHA butylated hydroxyanisole TFA trifluoroacetic acid MeCN acetonitrile |
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