牛磺酸上调基因1和微小RNA-145在胃癌中的表达关系研究

目的检测牛磺酸上调基因1（TUG1）和微小RNA-145（miR-145）在胃癌中的表达情况，探讨两者在胃癌发病中的作用及关系。 方法选取2016年2月至2017年2月于海南省肿瘤医院行根治性胃切除术的局部进展期胃癌患者110例，采用实时荧光定量-聚合酶链反应（RT-PCR）方法检测胃癌组织和癌旁正常组织中TUG1、miR-145的相对表达量，分析TUG1、miR-145表达相关性及其与临床病理资料、术后生存的关系。 结果与正常组织相比，胃癌组织中TUG1的表达明显升高（P<0.05），miR-145的表达明显降低（P<0.05）。胃癌组织中TUG1、miR-145的相对表达量呈明显负相关关系（P<0.05）。TUG1的表达水平与胃癌患者的淋巴结转移、肿瘤分化程度、临床分期有关（P<0.05），miR-145的表达水平与胃癌患者的淋巴结转移、临床分期有关（P<0.05）。TUG1低表达的胃癌患者总生存率明显高于TUG1高表达者（P=0.010），miR-145高表达胃癌患者总生存率明显高于miR-145低表达者（P<0.001）。 结论在胃癌组织中，TUG1明显高表达，miR-145明显低表达，且两者呈负相关关系，具有判断胃癌预后的价值。

Relationship of taurine up-regulated gene 1 and miRNA-145 in gastric cancer

ObjectiveTo detect the expression of taurine up-regulated gene 1 (TUG1) and microRNA-145 (miR-145) in gastric cancer, and to explore their role and relationship in the pathogenesis of gastric cancer. MethodsFrom February 2016 to February 2017, 110 cases of gastric cancer underwent radical operation in Hainan Cancer Hospital were selected, the gastric cancer tissues of the patients were taken as the study samples, and the normal tissues beside the cancer were taken as the control samples, the relative expressions of TUG1 and miR-145 in gastric cancer tissue and paracancerous normal tissue were detected by real-time fluorescence quantitative PCR. The relationships between the expressions of TUG1 and miR-145and the clinicopathological data, and the correlation between the relative expressions of TUG1 andmiR-145 and the postoperative survival were analyzed. ResultsCompared with normal tissues, the expression of TUG1 was significantly increased in gastric cancer tissues (P<0.05), the expression of miR-145decreased significantly (P<0.05). There was a negative correlation between the relative expression of TUG1 and miR-145 in gastric cancer (P<0.05). There were significant differences in lymph node metastasis, tumor differentiation and clinical stage between the TUG1 high expression group and the TUG1 low expression group (P<0.05). The expression level of miR-145 was related to lymph node metastasis and clinical stage of patients with gastric cancer (P<0.05). The overall survival rate of gastric cancer patients with TUG1 low expression was significantly higher than that of patients with TUG1 high expression(P=0.010), and the overall survival rate of gastric cancer patients with miR-145 low expression of was significantly lower than that of patients with miR-145 high expression (P<0.001). ConclusionTUG1 is significantly highly expressed in gastric cancer, miR-145 is significantly highly expressed in gastric cancer, they are negatively correlated, and have the value of predicting the prognosis of gastric cancer.